A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.

DNA repair was measured in an ionising radiation-sensitive mutant of a human bladder carcinoma cell line. No difference in the rate or extent of double-strand break rejoining was found using the techniques of neutral filter elution and pulsed-field gel electrophoresis. In contrast, significant diffe...

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Detalles Bibliográficos
Autores principales: Powell, S. N., Whitaker, S. J., Edwards, S. M., McMillan, T. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977752/
https://www.ncbi.nlm.nih.gov/pubmed/1616851
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author Powell, S. N.
Whitaker, S. J.
Edwards, S. M.
McMillan, T. J.
author_facet Powell, S. N.
Whitaker, S. J.
Edwards, S. M.
McMillan, T. J.
author_sort Powell, S. N.
collection PubMed
description DNA repair was measured in an ionising radiation-sensitive mutant of a human bladder carcinoma cell line. No difference in the rate or extent of double-strand break rejoining was found using the techniques of neutral filter elution and pulsed-field gel electrophoresis. In contrast, significant differences in repair fidelity, measured by plasmid reconstitution, were found. The parent line had a repair fidelity of 84.7% compared with 58.9% for S40b (P = 0.0003). It is suggested that repair fidelity can be an important determinant of radiosensitivity in human tumour cells.
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spelling pubmed-19777522009-09-10 A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line. Powell, S. N. Whitaker, S. J. Edwards, S. M. McMillan, T. J. Br J Cancer Research Article DNA repair was measured in an ionising radiation-sensitive mutant of a human bladder carcinoma cell line. No difference in the rate or extent of double-strand break rejoining was found using the techniques of neutral filter elution and pulsed-field gel electrophoresis. In contrast, significant differences in repair fidelity, measured by plasmid reconstitution, were found. The parent line had a repair fidelity of 84.7% compared with 58.9% for S40b (P = 0.0003). It is suggested that repair fidelity can be an important determinant of radiosensitivity in human tumour cells. Nature Publishing Group 1992-06 /pmc/articles/PMC1977752/ /pubmed/1616851 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Powell, S. N.
Whitaker, S. J.
Edwards, S. M.
McMillan, T. J.
A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.
title A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.
title_full A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.
title_fullStr A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.
title_full_unstemmed A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.
title_short A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.
title_sort dna repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977752/
https://www.ncbi.nlm.nih.gov/pubmed/1616851
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