Expression of c-erbB-2 protein in papillary thyroid carcinomas.

c-erbB-2 protein expression was investigated immunohistochemically in frozen thyroid tissue specimens from 42 patients using a polyclonal sheep antibody. c-erbB-2 immunoreactivity was detected in 12 out of 17 papillary carcinomas, while no c-erbB-2 protein immunostaining was seen in cases of follicu...

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Autores principales: Haugen, D. R., Akslen, L. A., Varhaug, J. E., Lillehaug, J. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977754/
https://www.ncbi.nlm.nih.gov/pubmed/1352125
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author Haugen, D. R.
Akslen, L. A.
Varhaug, J. E.
Lillehaug, J. R.
author_facet Haugen, D. R.
Akslen, L. A.
Varhaug, J. E.
Lillehaug, J. R.
author_sort Haugen, D. R.
collection PubMed
description c-erbB-2 protein expression was investigated immunohistochemically in frozen thyroid tissue specimens from 42 patients using a polyclonal sheep antibody. c-erbB-2 immunoreactivity was detected in 12 out of 17 papillary carcinomas, while no c-erbB-2 protein immunostaining was seen in cases of follicular adenoma (five cases), follicular carcinoma (five cases) or medullary carcinoma (one case), nor in cases of non-neoplastic tissue, including normal thyroid tissue from tumour-bearing glands. RNA was extracted from 51 thyroid tissue samples from 34 of the above patients, and c-erbB-2 mRNA was analysed by slot-blot hybridisation. c-erbB-2 mRNA was detectable in all samples, but papillary carcinomas and lymph node metastases showed significantly higher levels of c-erbB-2 mRNA compared to non-neoplastic tissue. The present demonstration of positive c-erbB-2 immunostaining in papillary thyroid carcinomas is contradictory to previous findings on formalin-fixed, paraffin-embedded material, and emphasises the importance of tissue quality for c-erbB-2 protein detection. IMAGES:
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spelling pubmed-19777542009-09-10 Expression of c-erbB-2 protein in papillary thyroid carcinomas. Haugen, D. R. Akslen, L. A. Varhaug, J. E. Lillehaug, J. R. Br J Cancer Research Article c-erbB-2 protein expression was investigated immunohistochemically in frozen thyroid tissue specimens from 42 patients using a polyclonal sheep antibody. c-erbB-2 immunoreactivity was detected in 12 out of 17 papillary carcinomas, while no c-erbB-2 protein immunostaining was seen in cases of follicular adenoma (five cases), follicular carcinoma (five cases) or medullary carcinoma (one case), nor in cases of non-neoplastic tissue, including normal thyroid tissue from tumour-bearing glands. RNA was extracted from 51 thyroid tissue samples from 34 of the above patients, and c-erbB-2 mRNA was analysed by slot-blot hybridisation. c-erbB-2 mRNA was detectable in all samples, but papillary carcinomas and lymph node metastases showed significantly higher levels of c-erbB-2 mRNA compared to non-neoplastic tissue. The present demonstration of positive c-erbB-2 immunostaining in papillary thyroid carcinomas is contradictory to previous findings on formalin-fixed, paraffin-embedded material, and emphasises the importance of tissue quality for c-erbB-2 protein detection. IMAGES: Nature Publishing Group 1992-06 /pmc/articles/PMC1977754/ /pubmed/1352125 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Haugen, D. R.
Akslen, L. A.
Varhaug, J. E.
Lillehaug, J. R.
Expression of c-erbB-2 protein in papillary thyroid carcinomas.
title Expression of c-erbB-2 protein in papillary thyroid carcinomas.
title_full Expression of c-erbB-2 protein in papillary thyroid carcinomas.
title_fullStr Expression of c-erbB-2 protein in papillary thyroid carcinomas.
title_full_unstemmed Expression of c-erbB-2 protein in papillary thyroid carcinomas.
title_short Expression of c-erbB-2 protein in papillary thyroid carcinomas.
title_sort expression of c-erbb-2 protein in papillary thyroid carcinomas.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977754/
https://www.ncbi.nlm.nih.gov/pubmed/1352125
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