Cargando…

Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma.

The prognostic value of flow cytometric parameters and tumour growth rate of melanoma metastases under the mouse renal capsule was investigated for tumours from 117 consecutive patients referred to the Helsinki University Central Hospital Melanoma Team. DNA flow cytometry (FCM) was interpretable for...

Descripción completa

Detalles Bibliográficos
Autores principales: Muhonen, T., Pyrhönen, S., Laasonen, A., Wasenius, V. M., Asko-Seljavaara, S., Franssila, K., Kangas, L.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977937/
https://www.ncbi.nlm.nih.gov/pubmed/1520590
_version_ 1782135369141059584
author Muhonen, T.
Pyrhönen, S.
Laasonen, A.
Wasenius, V. M.
Asko-Seljavaara, S.
Franssila, K.
Kangas, L.
author_facet Muhonen, T.
Pyrhönen, S.
Laasonen, A.
Wasenius, V. M.
Asko-Seljavaara, S.
Franssila, K.
Kangas, L.
author_sort Muhonen, T.
collection PubMed
description The prognostic value of flow cytometric parameters and tumour growth rate of melanoma metastases under the mouse renal capsule was investigated for tumours from 117 consecutive patients referred to the Helsinki University Central Hospital Melanoma Team. DNA flow cytometry (FCM) was interpretable for the tumours of 114 patients, and growth rate analysis for 82 patients, both results being available from 79 patients. Thirty-six percent of the tumours were DNA diploid and 64% DNA aneuploid. Tumour ploidy and S-phase fraction were shown by multivariate Cox model analysis to be independent prognostic variables and major determinants of survival after first recurrence. Patients with DNA diploid or aneuploid tumours survived a median 16 and 27 months, respectively. A high growth rate of tumour sample in vivo under the mouse renal capsule tended to be a sign of poor prognosis, although not reaching statistical significance. Combining the results of FCM, tumour growth rate and TNM stage, we propose a highly efficient prognostic scoring method. Patients with a score above 0.75 had a median survival of 11 months compared to 30 months among patients scoring under 0.75 (P less than 0.0001). This score was the most significant (P less than 0.0001) prognostic factor in the Cox model when TNM stage, age, ploidy, SPF, and tumour growth rate were analysed as covariates.
format Text
id pubmed-1977937
institution National Center for Biotechnology Information
language English
publishDate 1992
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-19779372009-09-10 Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma. Muhonen, T. Pyrhönen, S. Laasonen, A. Wasenius, V. M. Asko-Seljavaara, S. Franssila, K. Kangas, L. Br J Cancer Research Article The prognostic value of flow cytometric parameters and tumour growth rate of melanoma metastases under the mouse renal capsule was investigated for tumours from 117 consecutive patients referred to the Helsinki University Central Hospital Melanoma Team. DNA flow cytometry (FCM) was interpretable for the tumours of 114 patients, and growth rate analysis for 82 patients, both results being available from 79 patients. Thirty-six percent of the tumours were DNA diploid and 64% DNA aneuploid. Tumour ploidy and S-phase fraction were shown by multivariate Cox model analysis to be independent prognostic variables and major determinants of survival after first recurrence. Patients with DNA diploid or aneuploid tumours survived a median 16 and 27 months, respectively. A high growth rate of tumour sample in vivo under the mouse renal capsule tended to be a sign of poor prognosis, although not reaching statistical significance. Combining the results of FCM, tumour growth rate and TNM stage, we propose a highly efficient prognostic scoring method. Patients with a score above 0.75 had a median survival of 11 months compared to 30 months among patients scoring under 0.75 (P less than 0.0001). This score was the most significant (P less than 0.0001) prognostic factor in the Cox model when TNM stage, age, ploidy, SPF, and tumour growth rate were analysed as covariates. Nature Publishing Group 1992-09 /pmc/articles/PMC1977937/ /pubmed/1520590 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Muhonen, T.
Pyrhönen, S.
Laasonen, A.
Wasenius, V. M.
Asko-Seljavaara, S.
Franssila, K.
Kangas, L.
Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma.
title Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma.
title_full Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma.
title_fullStr Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma.
title_full_unstemmed Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma.
title_short Tumour growth rate and DNA flow cytometry parameters as prognostic factors in metastatic melanoma.
title_sort tumour growth rate and dna flow cytometry parameters as prognostic factors in metastatic melanoma.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977937/
https://www.ncbi.nlm.nih.gov/pubmed/1520590
work_keys_str_mv AT muhonent tumourgrowthrateanddnaflowcytometryparametersasprognosticfactorsinmetastaticmelanoma
AT pyrhonens tumourgrowthrateanddnaflowcytometryparametersasprognosticfactorsinmetastaticmelanoma
AT laasonena tumourgrowthrateanddnaflowcytometryparametersasprognosticfactorsinmetastaticmelanoma
AT waseniusvm tumourgrowthrateanddnaflowcytometryparametersasprognosticfactorsinmetastaticmelanoma
AT askoseljavaaras tumourgrowthrateanddnaflowcytometryparametersasprognosticfactorsinmetastaticmelanoma
AT franssilak tumourgrowthrateanddnaflowcytometryparametersasprognosticfactorsinmetastaticmelanoma
AT kangasl tumourgrowthrateanddnaflowcytometryparametersasprognosticfactorsinmetastaticmelanoma