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Glutathione S-transferase expression in the human testis and testicular germ cell neoplasia.

Glutathione S-transferase (GST) isoenzyme expression is altered in a variety of neoplasms and the enzymes are implicated in metabolism of carcinogens and resistance to drugs, including cisplatin. We have studied GST Alpha, Pi, Mu and microsomal isoenzyme expression by immunohistochemistry in normal...

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Autores principales: Klys, H. S., Whillis, D., Howard, G., Harrison, D. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977959/
https://www.ncbi.nlm.nih.gov/pubmed/1355663
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author Klys, H. S.
Whillis, D.
Howard, G.
Harrison, D. J.
author_facet Klys, H. S.
Whillis, D.
Howard, G.
Harrison, D. J.
author_sort Klys, H. S.
collection PubMed
description Glutathione S-transferase (GST) isoenzyme expression is altered in a variety of neoplasms and the enzymes are implicated in metabolism of carcinogens and resistance to drugs, including cisplatin. We have studied GST Alpha, Pi, Mu and microsomal isoenzyme expression by immunohistochemistry in normal and cryptorchid testes, intratubal germ cell neoplasia (ITGCN), seminoma and non-seminomatous germ cell tumours. In 16 stage II-IV malignant teratoma intermediate (MTI) both orchidectomy and post-treatment residual surgical masses were studied. All four isoenzymes were strongly expressed in Leydig and Sertoli cells. GST Pi was absent from normal spermatogonia but strongly expressed by the neoplastic germ cells of ITGCN and seminoma. GST Pi was strongly expressed in all elements of teratoma, irrespective of differentiation. There were no qualitative differences in expression between primary and post-chemotherapy metastases. GST Alpha expression in teratoma correlated with epithelial differentiation. GSTs may be important in normal spermatogenesis and protection of germ cells from teratogens and carcinogens. They may have a role in testicular tumour drug resistance but this role is not well defined. GST Pi is a new marker for ITGCN. IMAGES:
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spelling pubmed-19779592009-09-10 Glutathione S-transferase expression in the human testis and testicular germ cell neoplasia. Klys, H. S. Whillis, D. Howard, G. Harrison, D. J. Br J Cancer Research Article Glutathione S-transferase (GST) isoenzyme expression is altered in a variety of neoplasms and the enzymes are implicated in metabolism of carcinogens and resistance to drugs, including cisplatin. We have studied GST Alpha, Pi, Mu and microsomal isoenzyme expression by immunohistochemistry in normal and cryptorchid testes, intratubal germ cell neoplasia (ITGCN), seminoma and non-seminomatous germ cell tumours. In 16 stage II-IV malignant teratoma intermediate (MTI) both orchidectomy and post-treatment residual surgical masses were studied. All four isoenzymes were strongly expressed in Leydig and Sertoli cells. GST Pi was absent from normal spermatogonia but strongly expressed by the neoplastic germ cells of ITGCN and seminoma. GST Pi was strongly expressed in all elements of teratoma, irrespective of differentiation. There were no qualitative differences in expression between primary and post-chemotherapy metastases. GST Alpha expression in teratoma correlated with epithelial differentiation. GSTs may be important in normal spermatogenesis and protection of germ cells from teratogens and carcinogens. They may have a role in testicular tumour drug resistance but this role is not well defined. GST Pi is a new marker for ITGCN. IMAGES: Nature Publishing Group 1992-09 /pmc/articles/PMC1977959/ /pubmed/1355663 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Klys, H. S.
Whillis, D.
Howard, G.
Harrison, D. J.
Glutathione S-transferase expression in the human testis and testicular germ cell neoplasia.
title Glutathione S-transferase expression in the human testis and testicular germ cell neoplasia.
title_full Glutathione S-transferase expression in the human testis and testicular germ cell neoplasia.
title_fullStr Glutathione S-transferase expression in the human testis and testicular germ cell neoplasia.
title_full_unstemmed Glutathione S-transferase expression in the human testis and testicular germ cell neoplasia.
title_short Glutathione S-transferase expression in the human testis and testicular germ cell neoplasia.
title_sort glutathione s-transferase expression in the human testis and testicular germ cell neoplasia.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977959/
https://www.ncbi.nlm.nih.gov/pubmed/1355663
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