Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer.

DNA Ploidy and S phase fraction (SPF) were measured in Stage I and II breast cancers from patients with at least 8 years of follow-up, to assess the prognostic significance of these data. Disaggregated sections of formalin-fixed, paraffin-embedded tumour were analysed by flow cytometry. SPF was calc...

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Autores principales: Stanton, P. D., Cooke, T. G., Oakes, S. J., Winstanley, J., Holt, S., George, W. D., Murray, G. D.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977991/
https://www.ncbi.nlm.nih.gov/pubmed/1419638
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author Stanton, P. D.
Cooke, T. G.
Oakes, S. J.
Winstanley, J.
Holt, S.
George, W. D.
Murray, G. D.
author_facet Stanton, P. D.
Cooke, T. G.
Oakes, S. J.
Winstanley, J.
Holt, S.
George, W. D.
Murray, G. D.
author_sort Stanton, P. D.
collection PubMed
description DNA Ploidy and S phase fraction (SPF) were measured in Stage I and II breast cancers from patients with at least 8 years of follow-up, to assess the prognostic significance of these data. Disaggregated sections of formalin-fixed, paraffin-embedded tumour were analysed by flow cytometry. SPF was calculated using a rectangular model of S phase, after subtraction of background debris using an exponential model. 64% of tumours were DNA aneuploid. The median SPF was 4.5% for DNA diploid, and 10.9% for DNA aneuploid tumours. There were small reductions in survival at 10 years for DNA aneuploid tumours, and for tumours with above median SPF, but these were not statistically significant. The relative hazard for DNA aneuploid tumours was 1.20 (95% CI 0.81-1.76), and for high SPF was 1.31 (95% CI 0.87-1.98). Neither factor was statistically correlated with survival in multivariate analysis. Technical and theoretical factors limit the accuracy and reproducibility of flow cytometric data, and may explain the lack of prognostic information given.
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spelling pubmed-19779912009-09-10 Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer. Stanton, P. D. Cooke, T. G. Oakes, S. J. Winstanley, J. Holt, S. George, W. D. Murray, G. D. Br J Cancer Research Article DNA Ploidy and S phase fraction (SPF) were measured in Stage I and II breast cancers from patients with at least 8 years of follow-up, to assess the prognostic significance of these data. Disaggregated sections of formalin-fixed, paraffin-embedded tumour were analysed by flow cytometry. SPF was calculated using a rectangular model of S phase, after subtraction of background debris using an exponential model. 64% of tumours were DNA aneuploid. The median SPF was 4.5% for DNA diploid, and 10.9% for DNA aneuploid tumours. There were small reductions in survival at 10 years for DNA aneuploid tumours, and for tumours with above median SPF, but these were not statistically significant. The relative hazard for DNA aneuploid tumours was 1.20 (95% CI 0.81-1.76), and for high SPF was 1.31 (95% CI 0.87-1.98). Neither factor was statistically correlated with survival in multivariate analysis. Technical and theoretical factors limit the accuracy and reproducibility of flow cytometric data, and may explain the lack of prognostic information given. Nature Publishing Group 1992-11 /pmc/articles/PMC1977991/ /pubmed/1419638 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Stanton, P. D.
Cooke, T. G.
Oakes, S. J.
Winstanley, J.
Holt, S.
George, W. D.
Murray, G. D.
Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer.
title Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer.
title_full Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer.
title_fullStr Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer.
title_full_unstemmed Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer.
title_short Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer.
title_sort lack of prognostic significance of dna ploidy and s phase fraction in breast cancer.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977991/
https://www.ncbi.nlm.nih.gov/pubmed/1419638
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