Glutathione S-transferase activity and isoenzyme composition in benign ovarian tumours, untreated malignant ovarian tumours, and malignant ovarian tumours after platinum/cyclophosphamide chemotherapy.

Glutathione S-transferase (GST) isoenzyme composition, isoenzyme quantities and enzymatic activity were investigated in benign (n = 4) ovarian tumours and malignant ovarian tumours, before (n = 20) and after (n = 16) chemotherapy. Enzymatic activity of GST in cytosols was measured by determining 1-c...

Descripción completa

Detalles Bibliográficos
Autores principales: van der Zee, A. G., van Ommen, B., Meijer, C., Hollema, H., van Bladeren, P. J., de Vries, E. G.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1978003/
https://www.ncbi.nlm.nih.gov/pubmed/1419639
_version_ 1782135383858872320
author van der Zee, A. G.
van Ommen, B.
Meijer, C.
Hollema, H.
van Bladeren, P. J.
de Vries, E. G.
author_facet van der Zee, A. G.
van Ommen, B.
Meijer, C.
Hollema, H.
van Bladeren, P. J.
de Vries, E. G.
author_sort van der Zee, A. G.
collection PubMed
description Glutathione S-transferase (GST) isoenzyme composition, isoenzyme quantities and enzymatic activity were investigated in benign (n = 4) ovarian tumours and malignant ovarian tumours, before (n = 20) and after (n = 16) chemotherapy. Enzymatic activity of GST in cytosols was measured by determining 1-chloro-2,4-dinitrobenzene conjugation with glutathione, cytosolic GST subunits were determined by wide pore reversed phase HPLC, using a S-hexylglutathione-agarose affinity column, and isoelectric focussing. Both GST activity and GST pi amount were not related to histopathologic type, differentiation grade, or tumour volume index in untreated malignant tumours. GST isoenzyme patterns were identical in benign tumours and malignant tumours before and after platinum/cyclophosphamide chemotherapy, while GST pi was the predominant transferase. Mean GST activity and GST pi amount were decreased (P < 0.05) in malignant ovarian tumours after platinum/cyclophosphamide chemotherapy compared to untreated ovarian malignant tumours. No relation was found in untreated ovarian tumours between GST pi amount and response to platinum/cyclophosphamide chemotherapy. Thus, within the limitations of the current study no arguments were found for a role of GST in in vivo drug resistance of malignant ovarian tumours to platinum/cyclophosphamide chemotherapy.
format Text
id pubmed-1978003
institution National Center for Biotechnology Information
language English
publishDate 1992
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-19780032009-09-10 Glutathione S-transferase activity and isoenzyme composition in benign ovarian tumours, untreated malignant ovarian tumours, and malignant ovarian tumours after platinum/cyclophosphamide chemotherapy. van der Zee, A. G. van Ommen, B. Meijer, C. Hollema, H. van Bladeren, P. J. de Vries, E. G. Br J Cancer Research Article Glutathione S-transferase (GST) isoenzyme composition, isoenzyme quantities and enzymatic activity were investigated in benign (n = 4) ovarian tumours and malignant ovarian tumours, before (n = 20) and after (n = 16) chemotherapy. Enzymatic activity of GST in cytosols was measured by determining 1-chloro-2,4-dinitrobenzene conjugation with glutathione, cytosolic GST subunits were determined by wide pore reversed phase HPLC, using a S-hexylglutathione-agarose affinity column, and isoelectric focussing. Both GST activity and GST pi amount were not related to histopathologic type, differentiation grade, or tumour volume index in untreated malignant tumours. GST isoenzyme patterns were identical in benign tumours and malignant tumours before and after platinum/cyclophosphamide chemotherapy, while GST pi was the predominant transferase. Mean GST activity and GST pi amount were decreased (P < 0.05) in malignant ovarian tumours after platinum/cyclophosphamide chemotherapy compared to untreated ovarian malignant tumours. No relation was found in untreated ovarian tumours between GST pi amount and response to platinum/cyclophosphamide chemotherapy. Thus, within the limitations of the current study no arguments were found for a role of GST in in vivo drug resistance of malignant ovarian tumours to platinum/cyclophosphamide chemotherapy. Nature Publishing Group 1992-11 /pmc/articles/PMC1978003/ /pubmed/1419639 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
van der Zee, A. G.
van Ommen, B.
Meijer, C.
Hollema, H.
van Bladeren, P. J.
de Vries, E. G.
Glutathione S-transferase activity and isoenzyme composition in benign ovarian tumours, untreated malignant ovarian tumours, and malignant ovarian tumours after platinum/cyclophosphamide chemotherapy.
title Glutathione S-transferase activity and isoenzyme composition in benign ovarian tumours, untreated malignant ovarian tumours, and malignant ovarian tumours after platinum/cyclophosphamide chemotherapy.
title_full Glutathione S-transferase activity and isoenzyme composition in benign ovarian tumours, untreated malignant ovarian tumours, and malignant ovarian tumours after platinum/cyclophosphamide chemotherapy.
title_fullStr Glutathione S-transferase activity and isoenzyme composition in benign ovarian tumours, untreated malignant ovarian tumours, and malignant ovarian tumours after platinum/cyclophosphamide chemotherapy.
title_full_unstemmed Glutathione S-transferase activity and isoenzyme composition in benign ovarian tumours, untreated malignant ovarian tumours, and malignant ovarian tumours after platinum/cyclophosphamide chemotherapy.
title_short Glutathione S-transferase activity and isoenzyme composition in benign ovarian tumours, untreated malignant ovarian tumours, and malignant ovarian tumours after platinum/cyclophosphamide chemotherapy.
title_sort glutathione s-transferase activity and isoenzyme composition in benign ovarian tumours, untreated malignant ovarian tumours, and malignant ovarian tumours after platinum/cyclophosphamide chemotherapy.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1978003/
https://www.ncbi.nlm.nih.gov/pubmed/1419639
work_keys_str_mv AT vanderzeeag glutathionestransferaseactivityandisoenzymecompositioninbenignovariantumoursuntreatedmalignantovariantumoursandmalignantovariantumoursafterplatinumcyclophosphamidechemotherapy
AT vanommenb glutathionestransferaseactivityandisoenzymecompositioninbenignovariantumoursuntreatedmalignantovariantumoursandmalignantovariantumoursafterplatinumcyclophosphamidechemotherapy
AT meijerc glutathionestransferaseactivityandisoenzymecompositioninbenignovariantumoursuntreatedmalignantovariantumoursandmalignantovariantumoursafterplatinumcyclophosphamidechemotherapy
AT hollemah glutathionestransferaseactivityandisoenzymecompositioninbenignovariantumoursuntreatedmalignantovariantumoursandmalignantovariantumoursafterplatinumcyclophosphamidechemotherapy
AT vanbladerenpj glutathionestransferaseactivityandisoenzymecompositioninbenignovariantumoursuntreatedmalignantovariantumoursandmalignantovariantumoursafterplatinumcyclophosphamidechemotherapy
AT devrieseg glutathionestransferaseactivityandisoenzymecompositioninbenignovariantumoursuntreatedmalignantovariantumoursandmalignantovariantumoursafterplatinumcyclophosphamidechemotherapy

Ejemplares similares