Cargando…

Biomarker discovery for colon cancer using a 761 gene RT-PCR assay

BACKGROUND: Reverse transcription PCR (RT-PCR) is widely recognized to be the gold standard method for quantifying gene expression. Studies using RT-PCR technology as a discovery tool have historically been limited to relatively small gene sets compared to other gene expression platforms such as mic...

Descripción completa

Detalles Bibliográficos
Autores principales:	Clark-Langone, Kim M, Wu, Jenny Y, Sangli, Chithra, Chen, Angela, Snable, James L, Nguyen, Anhthu, Hackett, James R, Baker, Joffre, Yothers, Greg, Kim, Chungyeul, Cronin, Maureen T
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2007
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1994687/ https://www.ncbi.nlm.nih.gov/pubmed/17697383 http://dx.doi.org/10.1186/1471-2164-8-279

Ejemplares similares

Analytical validation of the Oncotype DX prostate cancer assay – a clinical RT-PCR assay optimized for prostate needle biopsies
por: Knezevic, Dejan, et al.
Publicado: (2013)

Translating tumor biology into personalized treatment planning: analytical performance characteristics of the Oncotype DX(® )Colon Cancer Assay
por: Clark-Langone, Kim M, et al.
Publicado: (2010)

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR
por: Zhang, Ni, et al.
Publicado: (2011)

Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI
por: Bester, Rachelle, et al.
Publicado: (2012)

Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
por: Varkonyi-Gasic, Erika, et al.
Publicado: (2007)

Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
por: Lu, Huanhuan, et al.
Publicado: (2021)

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
por: Long, Samuel, et al.
Publicado: (2021)

Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses
por: Davó, Laura, et al.
Publicado: (2020)

Selection of optimal reference genes for normalization in quantitative RT-PCR
por: Chervoneva, Inna, et al.
Publicado: (2010)

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors
por: Caldana, Camila, et al.
Publicado: (2007)

Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers
por: Maroniche, Guillermo A, et al.
Publicado: (2011)

Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR
por: Kuamsab, Napaporn, et al.
Publicado: (2012)

Development of a highly sensitive real-time one step RT-PCR combined complementary locked primer technology and conjugated minor groove binder probe
por: Hong, JiYoung, et al.
Publicado: (2011)

Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses
por: Saxena, Parag, et al.
Publicado: (2008)

A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition
por: Scipioni, Alexandra, et al.
Publicado: (2008)

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV)
por: Liu, Wenwen, et al.
Publicado: (2013)

Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies
por: Noutsias, Michel, et al.
Publicado: (2008)

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
por: Balcells, Ingrid, et al.
Publicado: (2011)

Data-driven normalization strategies for high-throughput quantitative RT-PCR
por: Mar, Jessica C, et al.
Publicado: (2009)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in Medicago truncatula
por: Kakar, Klementina, et al.
Publicado: (2008)

A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame
por: Yao, Ermei, et al.
Publicado: (2005)

Semiquantitative RT-PCR measurement of gene expression in rat tissues including a correction for varying cell size and number
por: Romero, Maria del Mar, et al.
Publicado: (2007)

Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT-PCR)
por: Provenzano, Maurizio, et al.
Publicado: (2003)

Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
por: Rodríguez-Mallon, Alina, et al.
Publicado: (2009)

Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey (Macaca mulatta) tissues
por: Ahn, Kung, et al.
Publicado: (2008)

A reliable method for quantification of splice variants using RT-qPCR
por: Camacho Londoño, Julia, et al.
Publicado: (2016)

An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development
por: Reid, Karen E, et al.
Publicado: (2006)

Comparison of molecular quantification of Plasmodium falciparum gametocytes by Pfs25 qRT-PCR and QT-NASBA in relation to mosquito infectivity
por: Pett, Helmi, et al.
Publicado: (2016)

Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay
por: Lyu, Xiaodong, et al.
Publicado: (2017)

Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos
por: Kuijk, Ewart W, et al.
Publicado: (2007)

Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process
por: Smith, Marjo V, et al.
Publicado: (2007)

Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
por: Valente, Valeria, et al.
Publicado: (2009)

A quantitative real-time RT-PCR assay for mature C. albicans biofilms
por: Xie, Zhihong, et al.
Publicado: (2011)

High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories
por: Summerauer, Andrea Maria, et al.
Publicado: (2018)

Standardization of Gene Expression Quantification by Absolute Real-Time qRT-PCR System Using a Single Standard for Marker and Reference Genes
por: Zhou, Yi-Hong, et al.
Publicado: (2010)

Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples
por: Zhang, Eileen Z, et al.
Publicado: (2013)

Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR
por: Berthet, Nicolas, et al.
Publicado: (2008)

Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis
por: Chen, Yongxin, et al.
Publicado: (2009)

A qRT-PCR assay for the expression of all Mal d 1 isoallergen genes
por: Pagliarani, Giulia, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_45fh28drvbve8irku5r4e5ti1u