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Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping
The Cre/loxP-system has become the system of choice for the generation of conditional so-called knockout mouse strains, i.e. the tissue-specific disruption of expression of a certain target gene. We here report the loss of expression of Cre recombinase in a transgenic mouse strain with increasing nu...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1995755/ https://www.ncbi.nlm.nih.gov/pubmed/17925861 http://dx.doi.org/10.1371/journal.pone.0001013 |
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author | Schulz, Tim J. Glaubitz, Markus Kuhlow, Doreen Thierbach, René Birringer, Marc Steinberg, Pablo Pfeiffer, Andreas F. H. Ristow, Michael |
author_facet | Schulz, Tim J. Glaubitz, Markus Kuhlow, Doreen Thierbach, René Birringer, Marc Steinberg, Pablo Pfeiffer, Andreas F. H. Ristow, Michael |
author_sort | Schulz, Tim J. |
collection | PubMed |
description | The Cre/loxP-system has become the system of choice for the generation of conditional so-called knockout mouse strains, i.e. the tissue-specific disruption of expression of a certain target gene. We here report the loss of expression of Cre recombinase in a transgenic mouse strain with increasing number of generations. This eventually led to the complete abrogation of gene expression of the inserted Cre cDNA while still being detectable at the genomic level. Conversely, loss of Cre expression caused an incomplete or even complete lack of disruption for the protein under investigation. As Cre expression in the tissue of interest in most cases cannot be addressed in vivo during the course of a study, our findings implicate the possibility that individual tail-biopsy genotypes may not necessarily indicate the presence or absence of gene disruption. This indicates that sustained post hoc analyses in regards to efficacy of disruption for every single study group member may be required. |
format | Text |
id | pubmed-1995755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-19957552007-10-10 Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping Schulz, Tim J. Glaubitz, Markus Kuhlow, Doreen Thierbach, René Birringer, Marc Steinberg, Pablo Pfeiffer, Andreas F. H. Ristow, Michael PLoS One Research Article The Cre/loxP-system has become the system of choice for the generation of conditional so-called knockout mouse strains, i.e. the tissue-specific disruption of expression of a certain target gene. We here report the loss of expression of Cre recombinase in a transgenic mouse strain with increasing number of generations. This eventually led to the complete abrogation of gene expression of the inserted Cre cDNA while still being detectable at the genomic level. Conversely, loss of Cre expression caused an incomplete or even complete lack of disruption for the protein under investigation. As Cre expression in the tissue of interest in most cases cannot be addressed in vivo during the course of a study, our findings implicate the possibility that individual tail-biopsy genotypes may not necessarily indicate the presence or absence of gene disruption. This indicates that sustained post hoc analyses in regards to efficacy of disruption for every single study group member may be required. Public Library of Science 2007-10-10 /pmc/articles/PMC1995755/ /pubmed/17925861 http://dx.doi.org/10.1371/journal.pone.0001013 Text en Schulz et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Schulz, Tim J. Glaubitz, Markus Kuhlow, Doreen Thierbach, René Birringer, Marc Steinberg, Pablo Pfeiffer, Andreas F. H. Ristow, Michael Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping |
title | Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping |
title_full | Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping |
title_fullStr | Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping |
title_full_unstemmed | Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping |
title_short | Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping |
title_sort | variable expression of cre recombinase transgenes precludes reliable prediction of tissue-specific gene disruption by tail-biopsy genotyping |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1995755/ https://www.ncbi.nlm.nih.gov/pubmed/17925861 http://dx.doi.org/10.1371/journal.pone.0001013 |
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