Cargando…
miRNA Profiling of Naïve, Effector and Memory CD8 T Cells
microRNAs have recently emerged as master regulators of gene expression during development and cell differentiation. Although profound changes in gene expression also occur during antigen-induced T cell differentiation, the role of miRNAs in the process is not known. We compared the miRNA expression...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2007
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2000354/ https://www.ncbi.nlm.nih.gov/pubmed/17925868 http://dx.doi.org/10.1371/journal.pone.0001020 |
_version_ | 1782135543553851392 |
---|---|
author | Wu, Haoquan Neilson, Joel R. Kumar, Priti Manocha, Monika Shankar, Premlata Sharp, Phillip A. Manjunath, N. |
author_facet | Wu, Haoquan Neilson, Joel R. Kumar, Priti Manocha, Monika Shankar, Premlata Sharp, Phillip A. Manjunath, N. |
author_sort | Wu, Haoquan |
collection | PubMed |
description | microRNAs have recently emerged as master regulators of gene expression during development and cell differentiation. Although profound changes in gene expression also occur during antigen-induced T cell differentiation, the role of miRNAs in the process is not known. We compared the miRNA expression profiles between antigen-specific naïve, effector and memory CD8+ T cells using 3 different methods-small RNA cloning, miRNA microarray analysis and real-time PCR. Although many miRNAs were expressed in all the T cell subsets, the frequency of 7 miRNAs (miR-16, miR-21, miR-142-3p, miR-142-5p, miR-150, miR-15b and let-7f) alone accounted for ∼60% of all miRNAs, and their expression was several fold higher than the other expressed miRNAs. Global downregulation of miRNAs (including 6/7 dominantly expressed miRNAs) was observed in effector T cells compared to naïve cells and the miRNA expression levels tended to come back up in memory T cells. However, a few miRNAs, notably miR-21 were higher in effector and memory T cells compared to naïve T cells. These results suggest that concomitant with profound changes in gene expression, miRNA profile also changes dynamically during T cell differentiation. Sequence analysis of the cloned mature miRNAs revealed an extensive degree of end polymorphism. While 3′end polymorphisms dominated, heterogeneity at both ends, resembling drosha/dicer processing shift was also seen in miR-142, suggesting a possible novel mechanism to generate new miRNA and/or to diversify miRNA target selection. Overall, our results suggest that dynamic changes in the expression of miRNAs may be important for the regulation of gene expression during antigen-induced T cell differentiation. Our study also suggests possible novel mechanisms for miRNA biogenesis and function. |
format | Text |
id | pubmed-2000354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-20003542007-10-10 miRNA Profiling of Naïve, Effector and Memory CD8 T Cells Wu, Haoquan Neilson, Joel R. Kumar, Priti Manocha, Monika Shankar, Premlata Sharp, Phillip A. Manjunath, N. PLoS One Research Article microRNAs have recently emerged as master regulators of gene expression during development and cell differentiation. Although profound changes in gene expression also occur during antigen-induced T cell differentiation, the role of miRNAs in the process is not known. We compared the miRNA expression profiles between antigen-specific naïve, effector and memory CD8+ T cells using 3 different methods-small RNA cloning, miRNA microarray analysis and real-time PCR. Although many miRNAs were expressed in all the T cell subsets, the frequency of 7 miRNAs (miR-16, miR-21, miR-142-3p, miR-142-5p, miR-150, miR-15b and let-7f) alone accounted for ∼60% of all miRNAs, and their expression was several fold higher than the other expressed miRNAs. Global downregulation of miRNAs (including 6/7 dominantly expressed miRNAs) was observed in effector T cells compared to naïve cells and the miRNA expression levels tended to come back up in memory T cells. However, a few miRNAs, notably miR-21 were higher in effector and memory T cells compared to naïve T cells. These results suggest that concomitant with profound changes in gene expression, miRNA profile also changes dynamically during T cell differentiation. Sequence analysis of the cloned mature miRNAs revealed an extensive degree of end polymorphism. While 3′end polymorphisms dominated, heterogeneity at both ends, resembling drosha/dicer processing shift was also seen in miR-142, suggesting a possible novel mechanism to generate new miRNA and/or to diversify miRNA target selection. Overall, our results suggest that dynamic changes in the expression of miRNAs may be important for the regulation of gene expression during antigen-induced T cell differentiation. Our study also suggests possible novel mechanisms for miRNA biogenesis and function. Public Library of Science 2007-10-10 /pmc/articles/PMC2000354/ /pubmed/17925868 http://dx.doi.org/10.1371/journal.pone.0001020 Text en Wu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wu, Haoquan Neilson, Joel R. Kumar, Priti Manocha, Monika Shankar, Premlata Sharp, Phillip A. Manjunath, N. miRNA Profiling of Naïve, Effector and Memory CD8 T Cells |
title | miRNA Profiling of Naïve, Effector and Memory CD8 T Cells |
title_full | miRNA Profiling of Naïve, Effector and Memory CD8 T Cells |
title_fullStr | miRNA Profiling of Naïve, Effector and Memory CD8 T Cells |
title_full_unstemmed | miRNA Profiling of Naïve, Effector and Memory CD8 T Cells |
title_short | miRNA Profiling of Naïve, Effector and Memory CD8 T Cells |
title_sort | mirna profiling of naïve, effector and memory cd8 t cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2000354/ https://www.ncbi.nlm.nih.gov/pubmed/17925868 http://dx.doi.org/10.1371/journal.pone.0001020 |
work_keys_str_mv | AT wuhaoquan mirnaprofilingofnaiveeffectorandmemorycd8tcells AT neilsonjoelr mirnaprofilingofnaiveeffectorandmemorycd8tcells AT kumarpriti mirnaprofilingofnaiveeffectorandmemorycd8tcells AT manochamonika mirnaprofilingofnaiveeffectorandmemorycd8tcells AT shankarpremlata mirnaprofilingofnaiveeffectorandmemorycd8tcells AT sharpphillipa mirnaprofilingofnaiveeffectorandmemorycd8tcells AT manjunathn mirnaprofilingofnaiveeffectorandmemorycd8tcells |