The anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol.

The effect of LiCl on melanoma cell growth and differentiation was studied in mouse and human melanoma cell lines. LiCl markedly inhibited B16 and HT-144 melanoma cell growth in vitro. Clonogenicity in soft agar of the melanoma cells was also markedly inhibited by LiCl. Pretreatment of B16 mouse mel...

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Autores principales: Nordenberg, J., Panet, C., Wasserman, L., Malik, Z., Fuchs, A., Stenzel, K. H., Novogrodsky, A.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1987
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001569/
https://www.ncbi.nlm.nih.gov/pubmed/3028460
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author Nordenberg, J.
Panet, C.
Wasserman, L.
Malik, Z.
Fuchs, A.
Stenzel, K. H.
Novogrodsky, A.
author_facet Nordenberg, J.
Panet, C.
Wasserman, L.
Malik, Z.
Fuchs, A.
Stenzel, K. H.
Novogrodsky, A.
author_sort Nordenberg, J.
collection PubMed
description The effect of LiCl on melanoma cell growth and differentiation was studied in mouse and human melanoma cell lines. LiCl markedly inhibited B16 and HT-144 melanoma cell growth in vitro. Clonogenicity in soft agar of the melanoma cells was also markedly inhibited by LiCl. Pretreatment of B16 mouse melanoma cells with LiCl delayed the appearance of melanoma tumours in syngeneic mice. Growth inhibition of cells was accompanied by morphological and biochemical alterations. LiCl induced cell enlargement and formation of dendrite-like structures. The activity of NADPH cytochrome c reductase, an enzymatic marker of endoplasmic reticulum was significantly (2-3 fold) increased. Addition of myo-inositol to cell cultures partially reversed the anti-proliferative and morphological effects of LiCl on melanoma cells. This finding may suggest that the anti-proliferative effect of LiCl is related to its effect on phosphatidylinositol metabolism. IMAGES:
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spelling pubmed-20015692009-09-10 The anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol. Nordenberg, J. Panet, C. Wasserman, L. Malik, Z. Fuchs, A. Stenzel, K. H. Novogrodsky, A. Br J Cancer Research Article The effect of LiCl on melanoma cell growth and differentiation was studied in mouse and human melanoma cell lines. LiCl markedly inhibited B16 and HT-144 melanoma cell growth in vitro. Clonogenicity in soft agar of the melanoma cells was also markedly inhibited by LiCl. Pretreatment of B16 mouse melanoma cells with LiCl delayed the appearance of melanoma tumours in syngeneic mice. Growth inhibition of cells was accompanied by morphological and biochemical alterations. LiCl induced cell enlargement and formation of dendrite-like structures. The activity of NADPH cytochrome c reductase, an enzymatic marker of endoplasmic reticulum was significantly (2-3 fold) increased. Addition of myo-inositol to cell cultures partially reversed the anti-proliferative and morphological effects of LiCl on melanoma cells. This finding may suggest that the anti-proliferative effect of LiCl is related to its effect on phosphatidylinositol metabolism. IMAGES: Nature Publishing Group 1987-01 /pmc/articles/PMC2001569/ /pubmed/3028460 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Nordenberg, J.
Panet, C.
Wasserman, L.
Malik, Z.
Fuchs, A.
Stenzel, K. H.
Novogrodsky, A.
The anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol.
title The anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol.
title_full The anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol.
title_fullStr The anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol.
title_full_unstemmed The anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol.
title_short The anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol.
title_sort anti-proliferative effect of lithium chloride on melanoma cells and its reversion by myo-inositol.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001569/
https://www.ncbi.nlm.nih.gov/pubmed/3028460
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