A novel technique for measuring human tissue pO2 at the cellular level.

Some electron-affinic drugs, developed as hypoxic cell radiosensitizers, become selectively bound to the molecules of hypoxic cells by metabolism. This technique has been used to identify zones of chronically hypoxic cells in multicellular spheroids and animal tumours. Tritiated-misonidazole was adm...

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Autores principales: Urtasun, R. C., Koch, C. J., Franko, A. J., Raleigh, J. A., Chapman, J. D.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1986
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001637/
https://www.ncbi.nlm.nih.gov/pubmed/3756081
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author Urtasun, R. C.
Koch, C. J.
Franko, A. J.
Raleigh, J. A.
Chapman, J. D.
author_facet Urtasun, R. C.
Koch, C. J.
Franko, A. J.
Raleigh, J. A.
Chapman, J. D.
author_sort Urtasun, R. C.
collection PubMed
description Some electron-affinic drugs, developed as hypoxic cell radiosensitizers, become selectively bound to the molecules of hypoxic cells by metabolism. This technique has been used to identify zones of chronically hypoxic cells in multicellular spheroids and animal tumours. Tritiated-misonidazole was administered to a patient with advanced melanoma 22 h prior to the surgical resection of a large metastatic s.c. lesion growing on the face. Autoradiographic analysis of histological sections revealed zones of intense labelling by the radioactive drug, indicative of tumour cells which were chronically hypoxic. This technique appears to provide an indirect measurement of tissue pO2 at the cellular level from which estimates of the tumour hypoxic fraction can be made. These data are encouraging as regards the development of 'sensitizer-adduct' procedures for the invasive and non-invasive measurement of hypoxia in both tumours and normal tissues. IMAGES:
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spelling pubmed-20016372009-09-10 A novel technique for measuring human tissue pO2 at the cellular level. Urtasun, R. C. Koch, C. J. Franko, A. J. Raleigh, J. A. Chapman, J. D. Br J Cancer Research Article Some electron-affinic drugs, developed as hypoxic cell radiosensitizers, become selectively bound to the molecules of hypoxic cells by metabolism. This technique has been used to identify zones of chronically hypoxic cells in multicellular spheroids and animal tumours. Tritiated-misonidazole was administered to a patient with advanced melanoma 22 h prior to the surgical resection of a large metastatic s.c. lesion growing on the face. Autoradiographic analysis of histological sections revealed zones of intense labelling by the radioactive drug, indicative of tumour cells which were chronically hypoxic. This technique appears to provide an indirect measurement of tissue pO2 at the cellular level from which estimates of the tumour hypoxic fraction can be made. These data are encouraging as regards the development of 'sensitizer-adduct' procedures for the invasive and non-invasive measurement of hypoxia in both tumours and normal tissues. IMAGES: Nature Publishing Group 1986-09 /pmc/articles/PMC2001637/ /pubmed/3756081 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Urtasun, R. C.
Koch, C. J.
Franko, A. J.
Raleigh, J. A.
Chapman, J. D.
A novel technique for measuring human tissue pO2 at the cellular level.
title A novel technique for measuring human tissue pO2 at the cellular level.
title_full A novel technique for measuring human tissue pO2 at the cellular level.
title_fullStr A novel technique for measuring human tissue pO2 at the cellular level.
title_full_unstemmed A novel technique for measuring human tissue pO2 at the cellular level.
title_short A novel technique for measuring human tissue pO2 at the cellular level.
title_sort novel technique for measuring human tissue po2 at the cellular level.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001637/
https://www.ncbi.nlm.nih.gov/pubmed/3756081
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