Comparative studies between a new human rhabdomyosarcoma cell line, JR-1 and its tumour of origin.

A new human embryonal rhabdomyosarcoma cell line, designated JR-1, is described that closely resembles the tumour from which it was derived. Comparative studies, by light and electron microscopy reveal morphological features such as myofibre formation, that are concordant with embryonal rhabdomyosar...

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Autores principales: Clayton, J., Pincott, J. R., van den Berghe, J. A., Kemshead, J. T.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1986
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001642/
https://www.ncbi.nlm.nih.gov/pubmed/3730258
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author Clayton, J.
Pincott, J. R.
van den Berghe, J. A.
Kemshead, J. T.
author_facet Clayton, J.
Pincott, J. R.
van den Berghe, J. A.
Kemshead, J. T.
author_sort Clayton, J.
collection PubMed
description A new human embryonal rhabdomyosarcoma cell line, designated JR-1, is described that closely resembles the tumour from which it was derived. Comparative studies, by light and electron microscopy reveal morphological features such as myofibre formation, that are concordant with embryonal rhabdomyosarcoma. Immunohistological investigations using a panel of monoclonal antibodies indicate that the cell surface antigen profile of the JR-1 cell line is similar to other embryonal rhabdomyosarcomas. In addition the cell line expresses the cytoplasmic intermediate filament protein desmin, only found in cells of rhabdoid origin. Karyotyping JR-1 shows the cells to contain variable numbers of chromosomes (range 44-100). DNA flow cytometry indicates that cells have an DNA content which is approximately twice normal. The JR-1 cell line has a doubling time of 29 h in culture and, in common with several other human cell lines, produces xenografts in nude mice within 6 weeks of inoculation. With detailed studies on the original tumour and the JR-1 cell line, the latter should prove an excellent model system for investigating the biology of rhabdomyosarcoma. IMAGES:
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spelling pubmed-20016422009-09-10 Comparative studies between a new human rhabdomyosarcoma cell line, JR-1 and its tumour of origin. Clayton, J. Pincott, J. R. van den Berghe, J. A. Kemshead, J. T. Br J Cancer Research Article A new human embryonal rhabdomyosarcoma cell line, designated JR-1, is described that closely resembles the tumour from which it was derived. Comparative studies, by light and electron microscopy reveal morphological features such as myofibre formation, that are concordant with embryonal rhabdomyosarcoma. Immunohistological investigations using a panel of monoclonal antibodies indicate that the cell surface antigen profile of the JR-1 cell line is similar to other embryonal rhabdomyosarcomas. In addition the cell line expresses the cytoplasmic intermediate filament protein desmin, only found in cells of rhabdoid origin. Karyotyping JR-1 shows the cells to contain variable numbers of chromosomes (range 44-100). DNA flow cytometry indicates that cells have an DNA content which is approximately twice normal. The JR-1 cell line has a doubling time of 29 h in culture and, in common with several other human cell lines, produces xenografts in nude mice within 6 weeks of inoculation. With detailed studies on the original tumour and the JR-1 cell line, the latter should prove an excellent model system for investigating the biology of rhabdomyosarcoma. IMAGES: Nature Publishing Group 1986-07 /pmc/articles/PMC2001642/ /pubmed/3730258 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Clayton, J.
Pincott, J. R.
van den Berghe, J. A.
Kemshead, J. T.
Comparative studies between a new human rhabdomyosarcoma cell line, JR-1 and its tumour of origin.
title Comparative studies between a new human rhabdomyosarcoma cell line, JR-1 and its tumour of origin.
title_full Comparative studies between a new human rhabdomyosarcoma cell line, JR-1 and its tumour of origin.
title_fullStr Comparative studies between a new human rhabdomyosarcoma cell line, JR-1 and its tumour of origin.
title_full_unstemmed Comparative studies between a new human rhabdomyosarcoma cell line, JR-1 and its tumour of origin.
title_short Comparative studies between a new human rhabdomyosarcoma cell line, JR-1 and its tumour of origin.
title_sort comparative studies between a new human rhabdomyosarcoma cell line, jr-1 and its tumour of origin.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001642/
https://www.ncbi.nlm.nih.gov/pubmed/3730258
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