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B-cell colony growth of malignant and normal B-cells.
B-cell colony growth of malignant and normal B-cells has been studied in a double layer (agar-fluid) colony assay. Stimulatory factors consisted of irradiated blood leukocytes, phytohaemagglutinin (PHA), interleukin 2 (IL2) and 12-0-tetradecanoylphorbol-13-acetate (TPA) in various combinations. B-ce...
| Autores principales: | , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
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Nature Publishing Group
1987
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001707/ https://www.ncbi.nlm.nih.gov/pubmed/3495283 |
| _version_ | 1782135659732926464 |
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| author | Kluin-Nelemans, J. C. Hakvoort, H. W. van Dierendonck, J. H. Beverstock, G. C. Fibbe, W. E. Willemze, R. van Rood, J. J. |
| author_facet | Kluin-Nelemans, J. C. Hakvoort, H. W. van Dierendonck, J. H. Beverstock, G. C. Fibbe, W. E. Willemze, R. van Rood, J. J. |
| author_sort | Kluin-Nelemans, J. C. |
| collection | PubMed |
| description | B-cell colony growth of malignant and normal B-cells has been studied in a double layer (agar-fluid) colony assay. Stimulatory factors consisted of irradiated blood leukocytes, phytohaemagglutinin (PHA), interleukin 2 (IL2) and 12-0-tetradecanoylphorbol-13-acetate (TPA) in various combinations. B-cell colonies have been obtained in all cases tested, i.e., 7/7 cases with chronic lymphocytic leukaemia, 7/7 cases with non-Hodgkin's lymphoma, 5/5 cases with hairy cell leukaemia and 7/7 normal B-cell suspensions, obtained from blood (X 3), bone marrow (X 2) and spleen (X 2). The plating efficacy ranged from 0.02-0.35, with a median of 0.07. Colony formation was found to be linear (r = 0.96) in the plating range of 0.5-8 X 10(5) cells. Secondary colonies could be obtained in 2 cases tested. DNA synthesizing cells in colonies were determined in 4 cases using monoclonal antibodies against DNA-incorporated bromodeoxyuridine (BrdUrd). In most cases the combination of PHA (with or without IL2) and irradiated leukocytes yielded the highest number of colonies, but in some experiments stimulation with TPA + IL2 was found to be optimal. IMAGES: |
| format | Text |
| id | pubmed-2001707 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1987 |
| publisher | Nature Publishing Group |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-20017072009-09-10 B-cell colony growth of malignant and normal B-cells. Kluin-Nelemans, J. C. Hakvoort, H. W. van Dierendonck, J. H. Beverstock, G. C. Fibbe, W. E. Willemze, R. van Rood, J. J. Br J Cancer Research Article B-cell colony growth of malignant and normal B-cells has been studied in a double layer (agar-fluid) colony assay. Stimulatory factors consisted of irradiated blood leukocytes, phytohaemagglutinin (PHA), interleukin 2 (IL2) and 12-0-tetradecanoylphorbol-13-acetate (TPA) in various combinations. B-cell colonies have been obtained in all cases tested, i.e., 7/7 cases with chronic lymphocytic leukaemia, 7/7 cases with non-Hodgkin's lymphoma, 5/5 cases with hairy cell leukaemia and 7/7 normal B-cell suspensions, obtained from blood (X 3), bone marrow (X 2) and spleen (X 2). The plating efficacy ranged from 0.02-0.35, with a median of 0.07. Colony formation was found to be linear (r = 0.96) in the plating range of 0.5-8 X 10(5) cells. Secondary colonies could be obtained in 2 cases tested. DNA synthesizing cells in colonies were determined in 4 cases using monoclonal antibodies against DNA-incorporated bromodeoxyuridine (BrdUrd). In most cases the combination of PHA (with or without IL2) and irradiated leukocytes yielded the highest number of colonies, but in some experiments stimulation with TPA + IL2 was found to be optimal. IMAGES: Nature Publishing Group 1987-04 /pmc/articles/PMC2001707/ /pubmed/3495283 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Research Article Kluin-Nelemans, J. C. Hakvoort, H. W. van Dierendonck, J. H. Beverstock, G. C. Fibbe, W. E. Willemze, R. van Rood, J. J. B-cell colony growth of malignant and normal B-cells. |
| title | B-cell colony growth of malignant and normal B-cells. |
| title_full | B-cell colony growth of malignant and normal B-cells. |
| title_fullStr | B-cell colony growth of malignant and normal B-cells. |
| title_full_unstemmed | B-cell colony growth of malignant and normal B-cells. |
| title_short | B-cell colony growth of malignant and normal B-cells. |
| title_sort | b-cell colony growth of malignant and normal b-cells. |
| topic | Research Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001707/ https://www.ncbi.nlm.nih.gov/pubmed/3495283 |
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