Detection of somatic changes in human cancer DNA by DNA fingerprint analysis.

Minisatellite DNA probes which can detect a large number of autosomal loci dispersed throughout the human genome were used to examine the constitutional and tumour DNA of 35 patients with a variety of cancers of which eight were of gastrointestinal origin. Somatic changes were seen in the tumour DNA...

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Autores principales: Thein, S. L., Jeffreys, A. J., Gooi, H. C., Cotter, F., Flint, J., O'Connor, N. T., Weatherall, D. J., Wainscoat, J. S.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1987
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001711/
https://www.ncbi.nlm.nih.gov/pubmed/3580260
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author Thein, S. L.
Jeffreys, A. J.
Gooi, H. C.
Cotter, F.
Flint, J.
O'Connor, N. T.
Weatherall, D. J.
Wainscoat, J. S.
author_facet Thein, S. L.
Jeffreys, A. J.
Gooi, H. C.
Cotter, F.
Flint, J.
O'Connor, N. T.
Weatherall, D. J.
Wainscoat, J. S.
author_sort Thein, S. L.
collection PubMed
description Minisatellite DNA probes which can detect a large number of autosomal loci dispersed throughout the human genome were used to examine the constitutional and tumour DNA of 35 patients with a variety of cancers of which eight were of gastrointestinal origin. Somatic changes were seen in the tumour DNA in ten of the 35 cases. The changes included alterations in the relative intensities of hybridising DNA fragments, and, in three cases of cancers of gastrointestinal origin, the appearance of novel minisatellite fragments not seen in the corresponding constitutional DNA. The results of this preliminary study suggests that DNA fingerprint analysis provides a useful technique for identifying somatic changes in cancers. IMAGES:
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spelling pubmed-20017112009-09-10 Detection of somatic changes in human cancer DNA by DNA fingerprint analysis. Thein, S. L. Jeffreys, A. J. Gooi, H. C. Cotter, F. Flint, J. O'Connor, N. T. Weatherall, D. J. Wainscoat, J. S. Br J Cancer Research Article Minisatellite DNA probes which can detect a large number of autosomal loci dispersed throughout the human genome were used to examine the constitutional and tumour DNA of 35 patients with a variety of cancers of which eight were of gastrointestinal origin. Somatic changes were seen in the tumour DNA in ten of the 35 cases. The changes included alterations in the relative intensities of hybridising DNA fragments, and, in three cases of cancers of gastrointestinal origin, the appearance of novel minisatellite fragments not seen in the corresponding constitutional DNA. The results of this preliminary study suggests that DNA fingerprint analysis provides a useful technique for identifying somatic changes in cancers. IMAGES: Nature Publishing Group 1987-04 /pmc/articles/PMC2001711/ /pubmed/3580260 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Thein, S. L.
Jeffreys, A. J.
Gooi, H. C.
Cotter, F.
Flint, J.
O'Connor, N. T.
Weatherall, D. J.
Wainscoat, J. S.
Detection of somatic changes in human cancer DNA by DNA fingerprint analysis.
title Detection of somatic changes in human cancer DNA by DNA fingerprint analysis.
title_full Detection of somatic changes in human cancer DNA by DNA fingerprint analysis.
title_fullStr Detection of somatic changes in human cancer DNA by DNA fingerprint analysis.
title_full_unstemmed Detection of somatic changes in human cancer DNA by DNA fingerprint analysis.
title_short Detection of somatic changes in human cancer DNA by DNA fingerprint analysis.
title_sort detection of somatic changes in human cancer dna by dna fingerprint analysis.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001711/
https://www.ncbi.nlm.nih.gov/pubmed/3580260
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