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Response of an ocular melanoma to subconjunctival injection of 5-thio-D-glucose or cis-platin.

Successful treatment of ocular tumours by chemotherapy and radiotherapy is sometimes limited by the special nature of the eye. Improvement is needed to avoid enucleation. Previous studies using locally administered antineoplastic agents have given promising results in the treatment of experimental o...

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Autores principales: Skov, K. A., Kumi, C., Rootman, J., Bussanich, N., Fellenz, M. O.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1987
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001735/
https://www.ncbi.nlm.nih.gov/pubmed/3606943
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author Skov, K. A.
Kumi, C.
Rootman, J.
Bussanich, N.
Fellenz, M. O.
author_facet Skov, K. A.
Kumi, C.
Rootman, J.
Bussanich, N.
Fellenz, M. O.
author_sort Skov, K. A.
collection PubMed
description Successful treatment of ocular tumours by chemotherapy and radiotherapy is sometimes limited by the special nature of the eye. Improvement is needed to avoid enucleation. Previous studies using locally administered antineoplastic agents have given promising results in the treatment of experimental ocular melanoma and spontaneous lymphoma. Another approach is use of radiosensitizers to improve radiotherapeutic effect. For the present study, 5-thio-D-glucose and cis-platin were chosen for evaluation in an ocular system because they exhibit antitumour activity, and interaction with radiation, particularly in hypoxia. Ocular absorption, toxicity, and pharmacokinetics after subconjunctival administration in rabbits were determined, and the effect of drug on tumours was measured using a Greene melanoma model. CHO cells were used for complimentary in vitro studies. 5-thio-D-glucose was readily absorbed into the eye (300 mg resulting in 5 mM in the aqueous) with no observable toxicity. When 5TG (300 mg) was administered at implantation, tumours were approximately half the size of controls. 5 mM 5TG is toxic to extremely hypoxic cells and gives measurable radiosensitization. Cis-platin levels as high as 0.68 microM were attained in the aqueous without local toxicity after 400 micrograms injection. This concentration causes toxicity in vitro. Cis-platin (400 mg) had a larger effect on tumour growth than 5TG given at, or one week after, implantation. Cis-platin may have potential for treatment of ocular tumours by local injection.
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spelling pubmed-20017352009-09-10 Response of an ocular melanoma to subconjunctival injection of 5-thio-D-glucose or cis-platin. Skov, K. A. Kumi, C. Rootman, J. Bussanich, N. Fellenz, M. O. Br J Cancer Research Article Successful treatment of ocular tumours by chemotherapy and radiotherapy is sometimes limited by the special nature of the eye. Improvement is needed to avoid enucleation. Previous studies using locally administered antineoplastic agents have given promising results in the treatment of experimental ocular melanoma and spontaneous lymphoma. Another approach is use of radiosensitizers to improve radiotherapeutic effect. For the present study, 5-thio-D-glucose and cis-platin were chosen for evaluation in an ocular system because they exhibit antitumour activity, and interaction with radiation, particularly in hypoxia. Ocular absorption, toxicity, and pharmacokinetics after subconjunctival administration in rabbits were determined, and the effect of drug on tumours was measured using a Greene melanoma model. CHO cells were used for complimentary in vitro studies. 5-thio-D-glucose was readily absorbed into the eye (300 mg resulting in 5 mM in the aqueous) with no observable toxicity. When 5TG (300 mg) was administered at implantation, tumours were approximately half the size of controls. 5 mM 5TG is toxic to extremely hypoxic cells and gives measurable radiosensitization. Cis-platin levels as high as 0.68 microM were attained in the aqueous without local toxicity after 400 micrograms injection. This concentration causes toxicity in vitro. Cis-platin (400 mg) had a larger effect on tumour growth than 5TG given at, or one week after, implantation. Cis-platin may have potential for treatment of ocular tumours by local injection. Nature Publishing Group 1987-05 /pmc/articles/PMC2001735/ /pubmed/3606943 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Skov, K. A.
Kumi, C.
Rootman, J.
Bussanich, N.
Fellenz, M. O.
Response of an ocular melanoma to subconjunctival injection of 5-thio-D-glucose or cis-platin.
title Response of an ocular melanoma to subconjunctival injection of 5-thio-D-glucose or cis-platin.
title_full Response of an ocular melanoma to subconjunctival injection of 5-thio-D-glucose or cis-platin.
title_fullStr Response of an ocular melanoma to subconjunctival injection of 5-thio-D-glucose or cis-platin.
title_full_unstemmed Response of an ocular melanoma to subconjunctival injection of 5-thio-D-glucose or cis-platin.
title_short Response of an ocular melanoma to subconjunctival injection of 5-thio-D-glucose or cis-platin.
title_sort response of an ocular melanoma to subconjunctival injection of 5-thio-d-glucose or cis-platin.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001735/
https://www.ncbi.nlm.nih.gov/pubmed/3606943
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