The effects of melphalan and misonidazole on the vasculature of a murine sarcoma.

A method for estimating both structural and functional vascular volumes in murine sarcomas is described. Intact vessels were demonstrated by the presence of laminin, a basement membrane-associated antigen, using an immunofluorescent technique, and functional vessels in the same sample by prior injec...

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Detalles Bibliográficos
Autores principales: Murray, J. C., Randhawa, V., Denekamp, J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1987
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001746/
https://www.ncbi.nlm.nih.gov/pubmed/3552014
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author Murray, J. C.
Randhawa, V.
Denekamp, J.
author_facet Murray, J. C.
Randhawa, V.
Denekamp, J.
author_sort Murray, J. C.
collection PubMed
description A method for estimating both structural and functional vascular volumes in murine sarcomas is described. Intact vessels were demonstrated by the presence of laminin, a basement membrane-associated antigen, using an immunofluorescent technique, and functional vessels in the same sample by prior injection with the DNA binding dye Hoechst 33342. No significant vascular effects were seen after melphalan but a very pronounced decrease in both functional and structural vascular volume was seen after MISO. Combined chemotherapy of a murine sarcoma with melphalan and MISO induced a rapid decrease in the functional vascular volume, and there was a resumption of blood flow prior to measurable regrowth. The fully regrown tumour retained the vascular characteristics of untreated tumours of similar size. IMAGES:
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spelling pubmed-20017462009-09-10 The effects of melphalan and misonidazole on the vasculature of a murine sarcoma. Murray, J. C. Randhawa, V. Denekamp, J. Br J Cancer Research Article A method for estimating both structural and functional vascular volumes in murine sarcomas is described. Intact vessels were demonstrated by the presence of laminin, a basement membrane-associated antigen, using an immunofluorescent technique, and functional vessels in the same sample by prior injection with the DNA binding dye Hoechst 33342. No significant vascular effects were seen after melphalan but a very pronounced decrease in both functional and structural vascular volume was seen after MISO. Combined chemotherapy of a murine sarcoma with melphalan and MISO induced a rapid decrease in the functional vascular volume, and there was a resumption of blood flow prior to measurable regrowth. The fully regrown tumour retained the vascular characteristics of untreated tumours of similar size. IMAGES: Nature Publishing Group 1987-03 /pmc/articles/PMC2001746/ /pubmed/3552014 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Murray, J. C.
Randhawa, V.
Denekamp, J.
The effects of melphalan and misonidazole on the vasculature of a murine sarcoma.
title The effects of melphalan and misonidazole on the vasculature of a murine sarcoma.
title_full The effects of melphalan and misonidazole on the vasculature of a murine sarcoma.
title_fullStr The effects of melphalan and misonidazole on the vasculature of a murine sarcoma.
title_full_unstemmed The effects of melphalan and misonidazole on the vasculature of a murine sarcoma.
title_short The effects of melphalan and misonidazole on the vasculature of a murine sarcoma.
title_sort effects of melphalan and misonidazole on the vasculature of a murine sarcoma.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001746/
https://www.ncbi.nlm.nih.gov/pubmed/3552014
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