A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma.

The proliferation rate of non-Hodgkin's lymphomas (NHL) was estimated by using 3 different methods. In cell suspension we determined the proportion of cells in cycle with the monoclonal antibody (Mab) Ki-67 and also in S-phase after the incorporation of bromo-deoxyuridine (BrdU) utilizing Mab a...

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Detalles Bibliográficos
Autores principales: Schrape, S., Jones, D. B., Wright, D. H.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1987
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001755/
https://www.ncbi.nlm.nih.gov/pubmed/3567062
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author Schrape, S.
Jones, D. B.
Wright, D. H.
author_facet Schrape, S.
Jones, D. B.
Wright, D. H.
author_sort Schrape, S.
collection PubMed
description The proliferation rate of non-Hodgkin's lymphomas (NHL) was estimated by using 3 different methods. In cell suspension we determined the proportion of cells in cycle with the monoclonal antibody (Mab) Ki-67 and also in S-phase after the incorporation of bromo-deoxyuridine (BrdU) utilizing Mab anti-BrdU. In low grade lymphomas 3.5 +/- 1.6% of the cells were in cycle and 1.2 +/- 0.9% in S-phase, the corresponding values for high grade lymphomas were 22.5 +/- 18.7% and 8.9 +/- 7.8% respectively. Frozen sections of NHL were reacted with an antibody to the transferrin receptor (TR) and Ki67 as markers for proliferative activity. A high number of TR positive cells was found in low grade lymphomas of all histological types, whereas Ki67 positivity correlated closely with grading. With a few exceptions, low grade lymphomas contained less than 25% Ki67 positive cells within the tumour cell population. This observation is relevant to treatment strategies for low grade NHL. IMAGES:
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spelling pubmed-20017552009-09-10 A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma. Schrape, S. Jones, D. B. Wright, D. H. Br J Cancer Research Article The proliferation rate of non-Hodgkin's lymphomas (NHL) was estimated by using 3 different methods. In cell suspension we determined the proportion of cells in cycle with the monoclonal antibody (Mab) Ki-67 and also in S-phase after the incorporation of bromo-deoxyuridine (BrdU) utilizing Mab anti-BrdU. In low grade lymphomas 3.5 +/- 1.6% of the cells were in cycle and 1.2 +/- 0.9% in S-phase, the corresponding values for high grade lymphomas were 22.5 +/- 18.7% and 8.9 +/- 7.8% respectively. Frozen sections of NHL were reacted with an antibody to the transferrin receptor (TR) and Ki67 as markers for proliferative activity. A high number of TR positive cells was found in low grade lymphomas of all histological types, whereas Ki67 positivity correlated closely with grading. With a few exceptions, low grade lymphomas contained less than 25% Ki67 positive cells within the tumour cell population. This observation is relevant to treatment strategies for low grade NHL. IMAGES: Nature Publishing Group 1987-03 /pmc/articles/PMC2001755/ /pubmed/3567062 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Schrape, S.
Jones, D. B.
Wright, D. H.
A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma.
title A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma.
title_full A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma.
title_fullStr A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma.
title_full_unstemmed A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma.
title_short A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma.
title_sort comparison of three methods for the determination of the growth fraction in non-hodgkin's lymphoma.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001755/
https://www.ncbi.nlm.nih.gov/pubmed/3567062
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