DNA analysis of breast tumour fine needle aspirates using flow cytometry.

Cellular DNA was analysed by flow cytometry in fine needles aspirates (FNA) from both benign and malignant breast lesions in order to determine the feasibility of flow cytometric analysis. In 22 of 26 (84%) benign and 69 of 74 (93%) malignant aspirates, sufficient cells were present to produce good...

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Autores principales: Levack, P. A., Mullen, P., Anderson, T. J., Miller, W. R., Forrest, A. P.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1987
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001890/
https://www.ncbi.nlm.nih.gov/pubmed/3426930
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author Levack, P. A.
Mullen, P.
Anderson, T. J.
Miller, W. R.
Forrest, A. P.
author_facet Levack, P. A.
Mullen, P.
Anderson, T. J.
Miller, W. R.
Forrest, A. P.
author_sort Levack, P. A.
collection PubMed
description Cellular DNA was analysed by flow cytometry in fine needles aspirates (FNA) from both benign and malignant breast lesions in order to determine the feasibility of flow cytometric analysis. In 22 of 26 (84%) benign and 69 of 74 (93%) malignant aspirates, sufficient cells were present to produce good quality DNA histograms. DNA in all 22 benign lesions was diploid. In contrast, of the 69 cancers with sufficient cells for analysis, 40.6% had a diploid DNA content alone, whilst 59.4% had an additional DNA aneuploid line. These results indicate that the majority of FNAs provide sufficient material for flow cytometric analysis of DNA profiles. Such aspirates taken in a sequential manner may also prove to be an ideal method of studying tumour response to therapy.
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spelling pubmed-20018902009-09-10 DNA analysis of breast tumour fine needle aspirates using flow cytometry. Levack, P. A. Mullen, P. Anderson, T. J. Miller, W. R. Forrest, A. P. Br J Cancer Research Article Cellular DNA was analysed by flow cytometry in fine needles aspirates (FNA) from both benign and malignant breast lesions in order to determine the feasibility of flow cytometric analysis. In 22 of 26 (84%) benign and 69 of 74 (93%) malignant aspirates, sufficient cells were present to produce good quality DNA histograms. DNA in all 22 benign lesions was diploid. In contrast, of the 69 cancers with sufficient cells for analysis, 40.6% had a diploid DNA content alone, whilst 59.4% had an additional DNA aneuploid line. These results indicate that the majority of FNAs provide sufficient material for flow cytometric analysis of DNA profiles. Such aspirates taken in a sequential manner may also prove to be an ideal method of studying tumour response to therapy. Nature Publishing Group 1987-11 /pmc/articles/PMC2001890/ /pubmed/3426930 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Levack, P. A.
Mullen, P.
Anderson, T. J.
Miller, W. R.
Forrest, A. P.
DNA analysis of breast tumour fine needle aspirates using flow cytometry.
title DNA analysis of breast tumour fine needle aspirates using flow cytometry.
title_full DNA analysis of breast tumour fine needle aspirates using flow cytometry.
title_fullStr DNA analysis of breast tumour fine needle aspirates using flow cytometry.
title_full_unstemmed DNA analysis of breast tumour fine needle aspirates using flow cytometry.
title_short DNA analysis of breast tumour fine needle aspirates using flow cytometry.
title_sort dna analysis of breast tumour fine needle aspirates using flow cytometry.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001890/
https://www.ncbi.nlm.nih.gov/pubmed/3426930
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