Preferentially expressed genes in stomach adenocarcinoma cells.

cDNA clones complementary to mRNA of neoplastic cells of human stomach tissue were used to examine quantitative changes in the mRNA levels of specific genes in neoplastic cells. Poly(A)+ RNA from poorly differentiated adenocarcinoma cells of a female patient with stomach cancer was used for construc...

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Detalles Bibliográficos
Autores principales: Shiosaka, T., Tanaka, Y., Kobayashi, Y.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1987
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001899/
https://www.ncbi.nlm.nih.gov/pubmed/3426917
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author Shiosaka, T.
Tanaka, Y.
Kobayashi, Y.
author_facet Shiosaka, T.
Tanaka, Y.
Kobayashi, Y.
author_sort Shiosaka, T.
collection PubMed
description cDNA clones complementary to mRNA of neoplastic cells of human stomach tissue were used to examine quantitative changes in the mRNA levels of specific genes in neoplastic cells. Poly(A)+ RNA from poorly differentiated adenocarcinoma cells of a female patient with stomach cancer was used for construction of a complementary DNA (cDNA) library. Screening of the 18,000 colonies utilizing 32P-cDNAs derived from normal human tissue and stomach carcinoma tissue samples was used to select clones likely to represent sequences preferentially expressed in stomach carcinoma cells. Twenty-six recombinants were initially selected and further analysis of these clones indicated that eight (4-3D, 9-2D, 9-4G, 29-1G, 29-6F, 37-1B, 115-5A and 52-5F) contain sequences preferentially expressed in stomach carcinoma cells. We have identified the 9-4G, 29-1A, and 29-6F genes which are differentially expressed in human neoplasia. IMAGES:
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spelling pubmed-20018992009-09-10 Preferentially expressed genes in stomach adenocarcinoma cells. Shiosaka, T. Tanaka, Y. Kobayashi, Y. Br J Cancer Research Article cDNA clones complementary to mRNA of neoplastic cells of human stomach tissue were used to examine quantitative changes in the mRNA levels of specific genes in neoplastic cells. Poly(A)+ RNA from poorly differentiated adenocarcinoma cells of a female patient with stomach cancer was used for construction of a complementary DNA (cDNA) library. Screening of the 18,000 colonies utilizing 32P-cDNAs derived from normal human tissue and stomach carcinoma tissue samples was used to select clones likely to represent sequences preferentially expressed in stomach carcinoma cells. Twenty-six recombinants were initially selected and further analysis of these clones indicated that eight (4-3D, 9-2D, 9-4G, 29-1G, 29-6F, 37-1B, 115-5A and 52-5F) contain sequences preferentially expressed in stomach carcinoma cells. We have identified the 9-4G, 29-1A, and 29-6F genes which are differentially expressed in human neoplasia. IMAGES: Nature Publishing Group 1987-11 /pmc/articles/PMC2001899/ /pubmed/3426917 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Shiosaka, T.
Tanaka, Y.
Kobayashi, Y.
Preferentially expressed genes in stomach adenocarcinoma cells.
title Preferentially expressed genes in stomach adenocarcinoma cells.
title_full Preferentially expressed genes in stomach adenocarcinoma cells.
title_fullStr Preferentially expressed genes in stomach adenocarcinoma cells.
title_full_unstemmed Preferentially expressed genes in stomach adenocarcinoma cells.
title_short Preferentially expressed genes in stomach adenocarcinoma cells.
title_sort preferentially expressed genes in stomach adenocarcinoma cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001899/
https://www.ncbi.nlm.nih.gov/pubmed/3426917
work_keys_str_mv AT shiosakat preferentiallyexpressedgenesinstomachadenocarcinomacells
AT tanakay preferentiallyexpressedgenesinstomachadenocarcinomacells
AT kobayashiy preferentiallyexpressedgenesinstomachadenocarcinomacells

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