X-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families.

The in vitro response to X-irradiation of cultured human fibroblasts was studied using a colony forming assay. A comprehensive reference range was established, giving a median D0 value of 98.5 cGy with an interquartile range of 86.5-110.5 cGy. Cells from 3 retinoblastoma family pedigrees were studie...

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Detalles Bibliográficos
Autores principales: Pledger, J. V., Craft, A. W., Bartlett, K., Long, D. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1987
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2002041/
https://www.ncbi.nlm.nih.gov/pubmed/3620303
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author Pledger, J. V.
Craft, A. W.
Bartlett, K.
Long, D. R.
author_facet Pledger, J. V.
Craft, A. W.
Bartlett, K.
Long, D. R.
author_sort Pledger, J. V.
collection PubMed
description The in vitro response to X-irradiation of cultured human fibroblasts was studied using a colony forming assay. A comprehensive reference range was established, giving a median D0 value of 98.5 cGy with an interquartile range of 86.5-110.5 cGy. Cells from 3 retinoblastoma family pedigrees were studied and the cell survival after exposure to X-rays was compared between affected (11 samples) and unaffected (26 samples) family members. No significant differences in response to ionising radiation were found between the controls, the affected and the unaffected members of the 3 families. The affected members had a median D0 of 97.5 cGy (interquartile range 87.5-107.5 cGy) and the unaffected members had a median D0 of 102 cGy (interquartile range 93-111 cGy). Thus radiosensitivity is not a useful marker for the detection of the retinoblastoma gene.
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spelling pubmed-20020412009-09-10 X-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families. Pledger, J. V. Craft, A. W. Bartlett, K. Long, D. R. Br J Cancer Research Article The in vitro response to X-irradiation of cultured human fibroblasts was studied using a colony forming assay. A comprehensive reference range was established, giving a median D0 value of 98.5 cGy with an interquartile range of 86.5-110.5 cGy. Cells from 3 retinoblastoma family pedigrees were studied and the cell survival after exposure to X-rays was compared between affected (11 samples) and unaffected (26 samples) family members. No significant differences in response to ionising radiation were found between the controls, the affected and the unaffected members of the 3 families. The affected members had a median D0 of 97.5 cGy (interquartile range 87.5-107.5 cGy) and the unaffected members had a median D0 of 102 cGy (interquartile range 93-111 cGy). Thus radiosensitivity is not a useful marker for the detection of the retinoblastoma gene. Nature Publishing Group 1987-06 /pmc/articles/PMC2002041/ /pubmed/3620303 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Pledger, J. V.
Craft, A. W.
Bartlett, K.
Long, D. R.
X-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families.
title X-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families.
title_full X-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families.
title_fullStr X-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families.
title_full_unstemmed X-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families.
title_short X-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families.
title_sort x-ray sensitivity of fibroblasts from patients with hereditary retinoblastoma and their families.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2002041/
https://www.ncbi.nlm.nih.gov/pubmed/3620303
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