Detection of human papillomavirus DNA in biopsies of human oral tissue.

We have employed molecular probes produced from DNA fragments of human papillomavirus, cloned into prokaryotic vectors, to detect virus nucleic acid sequences in extracts of human oral tissues. The study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had be...

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Autores principales: Maitland, N. J., Cox, M. F., Lynas, C., Prime, S. S., Meanwell, C. A., Scully, C.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1987
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2002211/
https://www.ncbi.nlm.nih.gov/pubmed/2822070
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author Maitland, N. J.
Cox, M. F.
Lynas, C.
Prime, S. S.
Meanwell, C. A.
Scully, C.
author_facet Maitland, N. J.
Cox, M. F.
Lynas, C.
Prime, S. S.
Meanwell, C. A.
Scully, C.
author_sort Maitland, N. J.
collection PubMed
description We have employed molecular probes produced from DNA fragments of human papillomavirus, cloned into prokaryotic vectors, to detect virus nucleic acid sequences in extracts of human oral tissues. The study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had been taken to accurately assess the pathology of each particular sample. The results show that a large proportion of the oral biopsies contained DNA which hybridized to the viral DNA probes, even under conditions of high stringency. The presence of virus did not correlate with neoplasia in the tissues examined, but HPV like sequences were found in a high proportion (80%) of biopsies taken from areas of keratosis and lichen planus and also in 41 to 46% of normal and tumour tissues. IMAGES:
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spelling pubmed-20022112009-09-10 Detection of human papillomavirus DNA in biopsies of human oral tissue. Maitland, N. J. Cox, M. F. Lynas, C. Prime, S. S. Meanwell, C. A. Scully, C. Br J Cancer Research Article We have employed molecular probes produced from DNA fragments of human papillomavirus, cloned into prokaryotic vectors, to detect virus nucleic acid sequences in extracts of human oral tissues. The study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had been taken to accurately assess the pathology of each particular sample. The results show that a large proportion of the oral biopsies contained DNA which hybridized to the viral DNA probes, even under conditions of high stringency. The presence of virus did not correlate with neoplasia in the tissues examined, but HPV like sequences were found in a high proportion (80%) of biopsies taken from areas of keratosis and lichen planus and also in 41 to 46% of normal and tumour tissues. IMAGES: Nature Publishing Group 1987-09 /pmc/articles/PMC2002211/ /pubmed/2822070 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Maitland, N. J.
Cox, M. F.
Lynas, C.
Prime, S. S.
Meanwell, C. A.
Scully, C.
Detection of human papillomavirus DNA in biopsies of human oral tissue.
title Detection of human papillomavirus DNA in biopsies of human oral tissue.
title_full Detection of human papillomavirus DNA in biopsies of human oral tissue.
title_fullStr Detection of human papillomavirus DNA in biopsies of human oral tissue.
title_full_unstemmed Detection of human papillomavirus DNA in biopsies of human oral tissue.
title_short Detection of human papillomavirus DNA in biopsies of human oral tissue.
title_sort detection of human papillomavirus dna in biopsies of human oral tissue.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2002211/
https://www.ncbi.nlm.nih.gov/pubmed/2822070
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