Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma

The indirect immunofluorescence technique demonstrated that human alpha fetoprotein (AFP) was present in a focal pattern in the cytoplasm of malignant liver cells of patients whose sera contained AFP. A few fibroblastlike cells in tissue culture of liver biopsies from patients with hepato-cellular c...

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Detalles Bibliográficos
Autores principales: Smith, J. A., Francis, T. I., Edington, G. M., Williams, A. O.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1971
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008448/
https://www.ncbi.nlm.nih.gov/pubmed/4329952
Descripción
Sumario:The indirect immunofluorescence technique demonstrated that human alpha fetoprotein (AFP) was present in a focal pattern in the cytoplasm of malignant liver cells of patients whose sera contained AFP. A few fibroblastlike cells in tissue culture of liver biopsies from patients with hepato-cellular carcinoma and AFP in their sera also had the protein. The intracellular localisation of human AFP was confirmed by centrifugation of washed, homogenised and ultra-sonically disrupted neonatal liver cells. Examination of livers of fetuses and neonates showed that AFP was present predominantly in the periportal parenchymal cells. IMAGES:

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