Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma

The indirect immunofluorescence technique demonstrated that human alpha fetoprotein (AFP) was present in a focal pattern in the cytoplasm of malignant liver cells of patients whose sera contained AFP. A few fibroblastlike cells in tissue culture of liver biopsies from patients with hepato-cellular c...

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Autores principales: Smith, J. A., Francis, T. I., Edington, G. M., Williams, A. O.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1971
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008448/
https://www.ncbi.nlm.nih.gov/pubmed/4329952
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author Smith, J. A.
Francis, T. I.
Edington, G. M.
Williams, A. O.
author_facet Smith, J. A.
Francis, T. I.
Edington, G. M.
Williams, A. O.
author_sort Smith, J. A.
collection PubMed
description The indirect immunofluorescence technique demonstrated that human alpha fetoprotein (AFP) was present in a focal pattern in the cytoplasm of malignant liver cells of patients whose sera contained AFP. A few fibroblastlike cells in tissue culture of liver biopsies from patients with hepato-cellular carcinoma and AFP in their sera also had the protein. The intracellular localisation of human AFP was confirmed by centrifugation of washed, homogenised and ultra-sonically disrupted neonatal liver cells. Examination of livers of fetuses and neonates showed that AFP was present predominantly in the periportal parenchymal cells. IMAGES:
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spelling pubmed-20084482009-09-10 Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma Smith, J. A. Francis, T. I. Edington, G. M. Williams, A. O. Br J Cancer Articles The indirect immunofluorescence technique demonstrated that human alpha fetoprotein (AFP) was present in a focal pattern in the cytoplasm of malignant liver cells of patients whose sera contained AFP. A few fibroblastlike cells in tissue culture of liver biopsies from patients with hepato-cellular carcinoma and AFP in their sera also had the protein. The intracellular localisation of human AFP was confirmed by centrifugation of washed, homogenised and ultra-sonically disrupted neonatal liver cells. Examination of livers of fetuses and neonates showed that AFP was present predominantly in the periportal parenchymal cells. IMAGES: Nature Publishing Group 1971-06 /pmc/articles/PMC2008448/ /pubmed/4329952 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Smith, J. A.
Francis, T. I.
Edington, G. M.
Williams, A. O.
Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma
title Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma
title_full Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma
title_fullStr Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma
title_full_unstemmed Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma
title_short Immunofluorescent Localisation of Human Alpha Fetoprotein in Fetal and Neonatal Livers and Cultured Cells from Hepatocellular Carcinoma
title_sort immunofluorescent localisation of human alpha fetoprotein in fetal and neonatal livers and cultured cells from hepatocellular carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008448/
https://www.ncbi.nlm.nih.gov/pubmed/4329952
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