The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography

The duration of the DNA synthesis period in normal and malignant human tissues from the larynx, trachea bronchus and esophagus of 13 patient has been estimated by a tritiated thymidine, carbon-14 thymidine double-labeling method in vitro. The DNA synthesis times range from approximately 10 to 25 hou...

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Autor principal: Fabrikant, J. I.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1970
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008511/
https://www.ncbi.nlm.nih.gov/pubmed/5428610
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author Fabrikant, J. I.
author_facet Fabrikant, J. I.
author_sort Fabrikant, J. I.
collection PubMed
description The duration of the DNA synthesis period in normal and malignant human tissues from the larynx, trachea bronchus and esophagus of 13 patient has been estimated by a tritiated thymidine, carbon-14 thymidine double-labeling method in vitro. The DNA synthesis times range from approximately 10 to 25 hours, are shortest in normal tissues, and are longer in malignant tumor cells than in benign tumor cells. The double-labeling method, the analysis of thymidine labeling index data for the measurement of the S period, and a model for determining potential doubling times in human tissues are described and discussed. IMAGES:
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spelling pubmed-20085112009-09-10 The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography Fabrikant, J. I. Br J Cancer Articles The duration of the DNA synthesis period in normal and malignant human tissues from the larynx, trachea bronchus and esophagus of 13 patient has been estimated by a tritiated thymidine, carbon-14 thymidine double-labeling method in vitro. The DNA synthesis times range from approximately 10 to 25 hours, are shortest in normal tissues, and are longer in malignant tumor cells than in benign tumor cells. The double-labeling method, the analysis of thymidine labeling index data for the measurement of the S period, and a model for determining potential doubling times in human tissues are described and discussed. IMAGES: Nature Publishing Group 1970-03 /pmc/articles/PMC2008511/ /pubmed/5428610 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Fabrikant, J. I.
The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography
title The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography
title_full The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography
title_fullStr The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography
title_full_unstemmed The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography
title_short The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography
title_sort kinetic of cellular proliferation in human tissues. determination of duration of dna synthesis using double labeling autoradiography
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008511/
https://www.ncbi.nlm.nih.gov/pubmed/5428610
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