Stimulation of Clonogenic Growth of Tumour Cells and Metastases in the Lungs by Local X-Radiation

Single cell suspensions of two allogeneic tumours (W-256 and Y-P388) injected intravenously produced macrocolonies in the lungs of rats. Colony forming efficiency (CFE, the number of colonies produced by each viable cell injected) was low in 6-week or older rats but was markedly increased by 1000-15...

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Autores principales: van den Brenk, H. A. S., Burch, W. M., Orton, C., Sharpington, C.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1973
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008788/
https://www.ncbi.nlm.nih.gov/pubmed/4701702
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author van den Brenk, H. A. S.
Burch, W. M.
Orton, C.
Sharpington, C.
author_facet van den Brenk, H. A. S.
Burch, W. M.
Orton, C.
Sharpington, C.
author_sort van den Brenk, H. A. S.
collection PubMed
description Single cell suspensions of two allogeneic tumours (W-256 and Y-P388) injected intravenously produced macrocolonies in the lungs of rats. Colony forming efficiency (CFE, the number of colonies produced by each viable cell injected) was low in 6-week or older rats but was markedly increased by 1000-1500 rad local thoracic irradiation (LTI) given 7-14 days before the tumour cell injection, or by antilymphocytic serum (ALS) but not by sublethal whole body irradiation (WBI). Similarly, LTI increased the incidence of pulmonary metastases produced by a solid tumour growing in the leg muscle. Stimulation of CFE by LTI was a strictly local phenomenon and not due to effects of irradiation on thymus, spleen or other tissues of the rat. LTI failed to increase CFE in immunized rats. It is concluded that (1) LTI stimulates clonogenic growth of tumour cells arrested in the lungs, by causing inflammatory reactions accompanied by regenerative cellular proliferation of lung tissue, which increases the “plating” efficiency of tumour cells, (2) the increase in CFE in lungs is not due to suppression of immunity to tumour growth by LTI. IMAGES:
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spelling pubmed-20087882009-09-10 Stimulation of Clonogenic Growth of Tumour Cells and Metastases in the Lungs by Local X-Radiation van den Brenk, H. A. S. Burch, W. M. Orton, C. Sharpington, C. Br J Cancer Articles Single cell suspensions of two allogeneic tumours (W-256 and Y-P388) injected intravenously produced macrocolonies in the lungs of rats. Colony forming efficiency (CFE, the number of colonies produced by each viable cell injected) was low in 6-week or older rats but was markedly increased by 1000-1500 rad local thoracic irradiation (LTI) given 7-14 days before the tumour cell injection, or by antilymphocytic serum (ALS) but not by sublethal whole body irradiation (WBI). Similarly, LTI increased the incidence of pulmonary metastases produced by a solid tumour growing in the leg muscle. Stimulation of CFE by LTI was a strictly local phenomenon and not due to effects of irradiation on thymus, spleen or other tissues of the rat. LTI failed to increase CFE in immunized rats. It is concluded that (1) LTI stimulates clonogenic growth of tumour cells arrested in the lungs, by causing inflammatory reactions accompanied by regenerative cellular proliferation of lung tissue, which increases the “plating” efficiency of tumour cells, (2) the increase in CFE in lungs is not due to suppression of immunity to tumour growth by LTI. IMAGES: Nature Publishing Group 1973-04 /pmc/articles/PMC2008788/ /pubmed/4701702 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
van den Brenk, H. A. S.
Burch, W. M.
Orton, C.
Sharpington, C.
Stimulation of Clonogenic Growth of Tumour Cells and Metastases in the Lungs by Local X-Radiation
title Stimulation of Clonogenic Growth of Tumour Cells and Metastases in the Lungs by Local X-Radiation
title_full Stimulation of Clonogenic Growth of Tumour Cells and Metastases in the Lungs by Local X-Radiation
title_fullStr Stimulation of Clonogenic Growth of Tumour Cells and Metastases in the Lungs by Local X-Radiation
title_full_unstemmed Stimulation of Clonogenic Growth of Tumour Cells and Metastases in the Lungs by Local X-Radiation
title_short Stimulation of Clonogenic Growth of Tumour Cells and Metastases in the Lungs by Local X-Radiation
title_sort stimulation of clonogenic growth of tumour cells and metastases in the lungs by local x-radiation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008788/
https://www.ncbi.nlm.nih.gov/pubmed/4701702
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