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The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata
Antibodies to the tumour-specific transplantation type antigen (TSTA) of a transplanted methylcholanthrene-induced sarcoma (MC-1) in syngeneic rats were studied using the techniques of indirect membrane immunofluorescence and mixed haemadsorption with a (51)Cr-labelled indicator cell. After tumour e...
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1973
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008825/ https://www.ncbi.nlm.nih.gov/pubmed/4568460 |
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author | Thomson, D. M. P. Steele, K. Alexander, P. |
author_facet | Thomson, D. M. P. Steele, K. Alexander, P. |
author_sort | Thomson, D. M. P. |
collection | PubMed |
description | Antibodies to the tumour-specific transplantation type antigen (TSTA) of a transplanted methylcholanthrene-induced sarcoma (MC-1) in syngeneic rats were studied using the techniques of indirect membrane immunofluorescence and mixed haemadsorption with a (51)Cr-labelled indicator cell. After tumour excision, anti-TSTA antibody was readily measurable in both serum and lymph. In contrast, the tumour-bearing animal had no measurable anti-TSTA antibody in the serum but low titres in the lymph. Consequently, we formed the hypothesis that in the presence of a growing tumour the serum contained antigen-antibody complexes with antigen in excess. To test this hypothesis, tumour-bearing serum was examined for the presence of free antigen and antigen-antibody complexes by 2 different methods. In the first method, tumour-bearing serum was cross-linked with glutaraldehyde and was found to absorb specifically the anti-TSTA antibody, indicating free circulating TSTA. Next, antigen-antibody complexes were split with salt or acid and separated into a low molecular weight (or “antigen”) fraction (<100,000) and a high molecular weight (or “antibody”) fraction (>100,000). The low M.W. fraction specifically inhibited the anti-TSTA antibody when tested by either membrane immunofluorescence or mixed haemadsorption, indicating the presence of antigen from antigen-antibody complexes in the tumour-bearing circulation. The possible effect on the host's immune response of circulating free tumour antigen and antigen-antibody complexes are discussed. |
format | Text |
id | pubmed-2008825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1973 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20088252009-09-10 The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata Thomson, D. M. P. Steele, K. Alexander, P. Br J Cancer Articles Antibodies to the tumour-specific transplantation type antigen (TSTA) of a transplanted methylcholanthrene-induced sarcoma (MC-1) in syngeneic rats were studied using the techniques of indirect membrane immunofluorescence and mixed haemadsorption with a (51)Cr-labelled indicator cell. After tumour excision, anti-TSTA antibody was readily measurable in both serum and lymph. In contrast, the tumour-bearing animal had no measurable anti-TSTA antibody in the serum but low titres in the lymph. Consequently, we formed the hypothesis that in the presence of a growing tumour the serum contained antigen-antibody complexes with antigen in excess. To test this hypothesis, tumour-bearing serum was examined for the presence of free antigen and antigen-antibody complexes by 2 different methods. In the first method, tumour-bearing serum was cross-linked with glutaraldehyde and was found to absorb specifically the anti-TSTA antibody, indicating free circulating TSTA. Next, antigen-antibody complexes were split with salt or acid and separated into a low molecular weight (or “antigen”) fraction (<100,000) and a high molecular weight (or “antibody”) fraction (>100,000). The low M.W. fraction specifically inhibited the anti-TSTA antibody when tested by either membrane immunofluorescence or mixed haemadsorption, indicating the presence of antigen from antigen-antibody complexes in the tumour-bearing circulation. The possible effect on the host's immune response of circulating free tumour antigen and antigen-antibody complexes are discussed. Nature Publishing Group 1973-01 /pmc/articles/PMC2008825/ /pubmed/4568460 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Articles Thomson, D. M. P. Steele, K. Alexander, P. The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata |
title | The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata |
title_full | The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata |
title_fullStr | The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata |
title_full_unstemmed | The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata |
title_short | The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata |
title_sort | presence of tumour specific membrane antigen in the serum of rats with chemically induced sarcomata |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008825/ https://www.ncbi.nlm.nih.gov/pubmed/4568460 |
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