Cargando…

The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata

Antibodies to the tumour-specific transplantation type antigen (TSTA) of a transplanted methylcholanthrene-induced sarcoma (MC-1) in syngeneic rats were studied using the techniques of indirect membrane immunofluorescence and mixed haemadsorption with a (51)Cr-labelled indicator cell. After tumour e...

Descripción completa

Detalles Bibliográficos
Autores principales: Thomson, D. M. P., Steele, K., Alexander, P.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1973
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008825/
https://www.ncbi.nlm.nih.gov/pubmed/4568460
_version_ 1782135993239863296
author Thomson, D. M. P.
Steele, K.
Alexander, P.
author_facet Thomson, D. M. P.
Steele, K.
Alexander, P.
author_sort Thomson, D. M. P.
collection PubMed
description Antibodies to the tumour-specific transplantation type antigen (TSTA) of a transplanted methylcholanthrene-induced sarcoma (MC-1) in syngeneic rats were studied using the techniques of indirect membrane immunofluorescence and mixed haemadsorption with a (51)Cr-labelled indicator cell. After tumour excision, anti-TSTA antibody was readily measurable in both serum and lymph. In contrast, the tumour-bearing animal had no measurable anti-TSTA antibody in the serum but low titres in the lymph. Consequently, we formed the hypothesis that in the presence of a growing tumour the serum contained antigen-antibody complexes with antigen in excess. To test this hypothesis, tumour-bearing serum was examined for the presence of free antigen and antigen-antibody complexes by 2 different methods. In the first method, tumour-bearing serum was cross-linked with glutaraldehyde and was found to absorb specifically the anti-TSTA antibody, indicating free circulating TSTA. Next, antigen-antibody complexes were split with salt or acid and separated into a low molecular weight (or “antigen”) fraction (<100,000) and a high molecular weight (or “antibody”) fraction (>100,000). The low M.W. fraction specifically inhibited the anti-TSTA antibody when tested by either membrane immunofluorescence or mixed haemadsorption, indicating the presence of antigen from antigen-antibody complexes in the tumour-bearing circulation. The possible effect on the host's immune response of circulating free tumour antigen and antigen-antibody complexes are discussed.
format Text
id pubmed-2008825
institution National Center for Biotechnology Information
language English
publishDate 1973
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-20088252009-09-10 The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata Thomson, D. M. P. Steele, K. Alexander, P. Br J Cancer Articles Antibodies to the tumour-specific transplantation type antigen (TSTA) of a transplanted methylcholanthrene-induced sarcoma (MC-1) in syngeneic rats were studied using the techniques of indirect membrane immunofluorescence and mixed haemadsorption with a (51)Cr-labelled indicator cell. After tumour excision, anti-TSTA antibody was readily measurable in both serum and lymph. In contrast, the tumour-bearing animal had no measurable anti-TSTA antibody in the serum but low titres in the lymph. Consequently, we formed the hypothesis that in the presence of a growing tumour the serum contained antigen-antibody complexes with antigen in excess. To test this hypothesis, tumour-bearing serum was examined for the presence of free antigen and antigen-antibody complexes by 2 different methods. In the first method, tumour-bearing serum was cross-linked with glutaraldehyde and was found to absorb specifically the anti-TSTA antibody, indicating free circulating TSTA. Next, antigen-antibody complexes were split with salt or acid and separated into a low molecular weight (or “antigen”) fraction (<100,000) and a high molecular weight (or “antibody”) fraction (>100,000). The low M.W. fraction specifically inhibited the anti-TSTA antibody when tested by either membrane immunofluorescence or mixed haemadsorption, indicating the presence of antigen from antigen-antibody complexes in the tumour-bearing circulation. The possible effect on the host's immune response of circulating free tumour antigen and antigen-antibody complexes are discussed. Nature Publishing Group 1973-01 /pmc/articles/PMC2008825/ /pubmed/4568460 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Thomson, D. M. P.
Steele, K.
Alexander, P.
The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata
title The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata
title_full The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata
title_fullStr The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata
title_full_unstemmed The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata
title_short The Presence of Tumour Specific Membrane Antigen in the Serum of Rats with Chemically Induced Sarcomata
title_sort presence of tumour specific membrane antigen in the serum of rats with chemically induced sarcomata
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008825/
https://www.ncbi.nlm.nih.gov/pubmed/4568460
work_keys_str_mv AT thomsondmp thepresenceoftumourspecificmembraneantigenintheserumofratswithchemicallyinducedsarcomata
AT steelek thepresenceoftumourspecificmembraneantigenintheserumofratswithchemicallyinducedsarcomata
AT alexanderp thepresenceoftumourspecificmembraneantigenintheserumofratswithchemicallyinducedsarcomata
AT thomsondmp presenceoftumourspecificmembraneantigenintheserumofratswithchemicallyinducedsarcomata
AT steelek presenceoftumourspecificmembraneantigenintheserumofratswithchemicallyinducedsarcomata
AT alexanderp presenceoftumourspecificmembraneantigenintheserumofratswithchemicallyinducedsarcomata