Cell Surface Antigenic Changes Induced in Normal Adult Rat Liver Cells by Carcinogen Treatment in vitro

Normal rat liver cell lines treated with the chemical carcinogen N-methyl-N-nitrosourea (MNU) elicited antibody production (detected by membrane immunofluorescence test in vitro) when injected into a highly inbred strain of rats from which the liver cells were originally isolated. In contrast, the c...

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Autores principales: Iype, P. T., Baldwin, R. W., Glaves, D.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1973
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008848/
https://www.ncbi.nlm.nih.gov/pubmed/4571664
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author Iype, P. T.
Baldwin, R. W.
Glaves, D.
author_facet Iype, P. T.
Baldwin, R. W.
Glaves, D.
author_sort Iype, P. T.
collection PubMed
description Normal rat liver cell lines treated with the chemical carcinogen N-methyl-N-nitrosourea (MNU) elicited antibody production (detected by membrane immunofluorescence test in vitro) when injected into a highly inbred strain of rats from which the liver cells were originally isolated. In contrast, the control cells which were untreated did not evoke humoral antibodies. Antisera raised against the MNU-treated cells reacted not only with the immunizing cells, but also with 3'-methyl-4-dimethyl aminoazobenzene and 3-methylcholanthrene-treated cells. However, this antiserum failed to react with cells treated with either aflatoxin or N-acetoxy-2-acetyl-aminofluorene. Embryonic antigens were found to be absent from the normal adult liver cell line. Preliminary results indicated that none could be detected on carcinogen-treated cells either.
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spelling pubmed-20088482009-09-10 Cell Surface Antigenic Changes Induced in Normal Adult Rat Liver Cells by Carcinogen Treatment in vitro Iype, P. T. Baldwin, R. W. Glaves, D. Br J Cancer Articles Normal rat liver cell lines treated with the chemical carcinogen N-methyl-N-nitrosourea (MNU) elicited antibody production (detected by membrane immunofluorescence test in vitro) when injected into a highly inbred strain of rats from which the liver cells were originally isolated. In contrast, the control cells which were untreated did not evoke humoral antibodies. Antisera raised against the MNU-treated cells reacted not only with the immunizing cells, but also with 3'-methyl-4-dimethyl aminoazobenzene and 3-methylcholanthrene-treated cells. However, this antiserum failed to react with cells treated with either aflatoxin or N-acetoxy-2-acetyl-aminofluorene. Embryonic antigens were found to be absent from the normal adult liver cell line. Preliminary results indicated that none could be detected on carcinogen-treated cells either. Nature Publishing Group 1973-02 /pmc/articles/PMC2008848/ /pubmed/4571664 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Iype, P. T.
Baldwin, R. W.
Glaves, D.
Cell Surface Antigenic Changes Induced in Normal Adult Rat Liver Cells by Carcinogen Treatment in vitro
title Cell Surface Antigenic Changes Induced in Normal Adult Rat Liver Cells by Carcinogen Treatment in vitro
title_full Cell Surface Antigenic Changes Induced in Normal Adult Rat Liver Cells by Carcinogen Treatment in vitro
title_fullStr Cell Surface Antigenic Changes Induced in Normal Adult Rat Liver Cells by Carcinogen Treatment in vitro
title_full_unstemmed Cell Surface Antigenic Changes Induced in Normal Adult Rat Liver Cells by Carcinogen Treatment in vitro
title_short Cell Surface Antigenic Changes Induced in Normal Adult Rat Liver Cells by Carcinogen Treatment in vitro
title_sort cell surface antigenic changes induced in normal adult rat liver cells by carcinogen treatment in vitro
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008848/
https://www.ncbi.nlm.nih.gov/pubmed/4571664
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AT glavesd cellsurfaceantigenicchangesinducedinnormaladultratlivercellsbycarcinogentreatmentinvitro

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