Cytochemistry, Cytogenetics and Ultrastructure of Hamster Tumour Cells Carrying Mouse Sarcoma Viral Genome (HT-1 Cells)

The pleomorphic cytology of the HT-1 cell line is described. Cytochemical studies indicated the presence of glycogen and lysosomes in these cells. Cytogenetic studies demonstrated the presence of a large telocentric chromosome (M1) and two minute chromosomes (M2) not found in normal hamster cells. A...

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Autores principales: Karpas, A., Cawley, J., Tuckerman, E., Flemans, R., Hayhoe, F. G. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1971
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008861/
https://www.ncbi.nlm.nih.gov/pubmed/4335635
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author Karpas, A.
Cawley, J.
Tuckerman, E.
Flemans, R.
Hayhoe, F. G. J.
author_facet Karpas, A.
Cawley, J.
Tuckerman, E.
Flemans, R.
Hayhoe, F. G. J.
author_sort Karpas, A.
collection PubMed
description The pleomorphic cytology of the HT-1 cell line is described. Cytochemical studies indicated the presence of glycogen and lysosomes in these cells. Cytogenetic studies demonstrated the presence of a large telocentric chromosome (M1) and two minute chromosomes (M2) not found in normal hamster cells. A cloned line was isolated which did not contain these marker chromosomes. All cells were hyperdiploid with chromosome number around triploidy, although none was a true triploid. Ultrastructural studies revealed the presence of “nuclear bodies”, “dense bodies” and “interchromatinic granules” which have been previously described in viral induced malignancies. A few of the cells contained in their cytoplasm structures of approximately 700 [unk] in diameter which bore some resemblance to immature virus particles. Both light and electron microscopy demonstrated some nuclei lacking chromatin aggregates. This is interpreted to mean that the total genetic material of these nuclei was dispersed as free DNA not linked with histone to form chromatin aggregates. IMAGES:
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spelling pubmed-20088612009-09-10 Cytochemistry, Cytogenetics and Ultrastructure of Hamster Tumour Cells Carrying Mouse Sarcoma Viral Genome (HT-1 Cells) Karpas, A. Cawley, J. Tuckerman, E. Flemans, R. Hayhoe, F. G. J. Br J Cancer Articles The pleomorphic cytology of the HT-1 cell line is described. Cytochemical studies indicated the presence of glycogen and lysosomes in these cells. Cytogenetic studies demonstrated the presence of a large telocentric chromosome (M1) and two minute chromosomes (M2) not found in normal hamster cells. A cloned line was isolated which did not contain these marker chromosomes. All cells were hyperdiploid with chromosome number around triploidy, although none was a true triploid. Ultrastructural studies revealed the presence of “nuclear bodies”, “dense bodies” and “interchromatinic granules” which have been previously described in viral induced malignancies. A few of the cells contained in their cytoplasm structures of approximately 700 [unk] in diameter which bore some resemblance to immature virus particles. Both light and electron microscopy demonstrated some nuclei lacking chromatin aggregates. This is interpreted to mean that the total genetic material of these nuclei was dispersed as free DNA not linked with histone to form chromatin aggregates. IMAGES: Nature Publishing Group 1971-12 /pmc/articles/PMC2008861/ /pubmed/4335635 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Karpas, A.
Cawley, J.
Tuckerman, E.
Flemans, R.
Hayhoe, F. G. J.
Cytochemistry, Cytogenetics and Ultrastructure of Hamster Tumour Cells Carrying Mouse Sarcoma Viral Genome (HT-1 Cells)
title Cytochemistry, Cytogenetics and Ultrastructure of Hamster Tumour Cells Carrying Mouse Sarcoma Viral Genome (HT-1 Cells)
title_full Cytochemistry, Cytogenetics and Ultrastructure of Hamster Tumour Cells Carrying Mouse Sarcoma Viral Genome (HT-1 Cells)
title_fullStr Cytochemistry, Cytogenetics and Ultrastructure of Hamster Tumour Cells Carrying Mouse Sarcoma Viral Genome (HT-1 Cells)
title_full_unstemmed Cytochemistry, Cytogenetics and Ultrastructure of Hamster Tumour Cells Carrying Mouse Sarcoma Viral Genome (HT-1 Cells)
title_short Cytochemistry, Cytogenetics and Ultrastructure of Hamster Tumour Cells Carrying Mouse Sarcoma Viral Genome (HT-1 Cells)
title_sort cytochemistry, cytogenetics and ultrastructure of hamster tumour cells carrying mouse sarcoma viral genome (ht-1 cells)
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008861/
https://www.ncbi.nlm.nih.gov/pubmed/4335635
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