A Thin-layer Gel Filtration Assay of Cytoplasmic Oestrogen Receptors. A Possible Screening Method for Hormone Dependent Tumours

A thin-layer gel filtration system for the assay of oestrogen receptors in target organ tissue samples as small as 50 mg is presented and compared with the sucrose gradient centrifugation method. Only specific high-affinity binding of [(3)H]17β-oestradiol to endometrial cytosol has been observed wit...

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Autores principales: Gore-Langton, R. E., Ashwood-Smith, M. J., Algard, F. T., van Netten, J. P.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1973
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008895/
https://www.ncbi.nlm.nih.gov/pubmed/4357271
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author Gore-Langton, R. E.
Ashwood-Smith, M. J.
Algard, F. T.
van Netten, J. P.
author_facet Gore-Langton, R. E.
Ashwood-Smith, M. J.
Algard, F. T.
van Netten, J. P.
author_sort Gore-Langton, R. E.
collection PubMed
description A thin-layer gel filtration system for the assay of oestrogen receptors in target organ tissue samples as small as 50 mg is presented and compared with the sucrose gradient centrifugation method. Only specific high-affinity binding of [(3)H]17β-oestradiol to endometrial cytosol has been observed with the thin-layer gel filtration assay, despite the presence of relatively high levels of nonspecific binding components. The system could be adapted to the clinical determination of oestrogen receptor content in biopsy material from mammary and endometrial tumours and would be of value in predicting hormone dependency.
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spelling pubmed-20088952009-09-10 A Thin-layer Gel Filtration Assay of Cytoplasmic Oestrogen Receptors. A Possible Screening Method for Hormone Dependent Tumours Gore-Langton, R. E. Ashwood-Smith, M. J. Algard, F. T. van Netten, J. P. Br J Cancer Articles A thin-layer gel filtration system for the assay of oestrogen receptors in target organ tissue samples as small as 50 mg is presented and compared with the sucrose gradient centrifugation method. Only specific high-affinity binding of [(3)H]17β-oestradiol to endometrial cytosol has been observed with the thin-layer gel filtration assay, despite the presence of relatively high levels of nonspecific binding components. The system could be adapted to the clinical determination of oestrogen receptor content in biopsy material from mammary and endometrial tumours and would be of value in predicting hormone dependency. Nature Publishing Group 1973-10 /pmc/articles/PMC2008895/ /pubmed/4357271 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Gore-Langton, R. E.
Ashwood-Smith, M. J.
Algard, F. T.
van Netten, J. P.
A Thin-layer Gel Filtration Assay of Cytoplasmic Oestrogen Receptors. A Possible Screening Method for Hormone Dependent Tumours
title A Thin-layer Gel Filtration Assay of Cytoplasmic Oestrogen Receptors. A Possible Screening Method for Hormone Dependent Tumours
title_full A Thin-layer Gel Filtration Assay of Cytoplasmic Oestrogen Receptors. A Possible Screening Method for Hormone Dependent Tumours
title_fullStr A Thin-layer Gel Filtration Assay of Cytoplasmic Oestrogen Receptors. A Possible Screening Method for Hormone Dependent Tumours
title_full_unstemmed A Thin-layer Gel Filtration Assay of Cytoplasmic Oestrogen Receptors. A Possible Screening Method for Hormone Dependent Tumours
title_short A Thin-layer Gel Filtration Assay of Cytoplasmic Oestrogen Receptors. A Possible Screening Method for Hormone Dependent Tumours
title_sort thin-layer gel filtration assay of cytoplasmic oestrogen receptors. a possible screening method for hormone dependent tumours
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008895/
https://www.ncbi.nlm.nih.gov/pubmed/4357271
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