The Histological Appearance of Tumours Derived from Rat Embryo Cells Transformed in vitro Spontaneously and After Treatment with Nitrosomethylurea

Wistar rat embryo cells were treated in vitro with either 25 μg/ml of nitrosomethylurea (NMU) or phosphate buffered saline. Both groups showed morphological transformation by the 13th passage but their ability to grow in soft agar did not occur until at least passage 23; plating efficiencies indicat...

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Detalles Bibliográficos
Autores principales: Kirkland, D. J., Pick, C. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1973
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008912/
https://www.ncbi.nlm.nih.gov/pubmed/4758371
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author Kirkland, D. J.
Pick, C. R.
author_facet Kirkland, D. J.
Pick, C. R.
author_sort Kirkland, D. J.
collection PubMed
description Wistar rat embryo cells were treated in vitro with either 25 μg/ml of nitrosomethylurea (NMU) or phosphate buffered saline. Both groups showed morphological transformation by the 13th passage but their ability to grow in soft agar did not occur until at least passage 23; plating efficiencies indicated that NMU had reduced transformation. However, both control and treated cells gave rise to fibrosarcomata after similar latent periods following inoculation into syngeneic recipients. The fibrosarcomata had “myxoid” and “leiomyomatous” areas, and two resembled haemangiopericytomata; for the most part the tumours were transplantable. Inoculation of cloned NMU-treated cells produced fibrosarcomata with a high proportion of giant cells but only after a very long latent period. No virus particles were detected in tumour samples by electron microscopy. IMAGES:
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spelling pubmed-20089122009-09-10 The Histological Appearance of Tumours Derived from Rat Embryo Cells Transformed in vitro Spontaneously and After Treatment with Nitrosomethylurea Kirkland, D. J. Pick, C. R. Br J Cancer Articles Wistar rat embryo cells were treated in vitro with either 25 μg/ml of nitrosomethylurea (NMU) or phosphate buffered saline. Both groups showed morphological transformation by the 13th passage but their ability to grow in soft agar did not occur until at least passage 23; plating efficiencies indicated that NMU had reduced transformation. However, both control and treated cells gave rise to fibrosarcomata after similar latent periods following inoculation into syngeneic recipients. The fibrosarcomata had “myxoid” and “leiomyomatous” areas, and two resembled haemangiopericytomata; for the most part the tumours were transplantable. Inoculation of cloned NMU-treated cells produced fibrosarcomata with a high proportion of giant cells but only after a very long latent period. No virus particles were detected in tumour samples by electron microscopy. IMAGES: Nature Publishing Group 1973-11 /pmc/articles/PMC2008912/ /pubmed/4758371 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Kirkland, D. J.
Pick, C. R.
The Histological Appearance of Tumours Derived from Rat Embryo Cells Transformed in vitro Spontaneously and After Treatment with Nitrosomethylurea
title The Histological Appearance of Tumours Derived from Rat Embryo Cells Transformed in vitro Spontaneously and After Treatment with Nitrosomethylurea
title_full The Histological Appearance of Tumours Derived from Rat Embryo Cells Transformed in vitro Spontaneously and After Treatment with Nitrosomethylurea
title_fullStr The Histological Appearance of Tumours Derived from Rat Embryo Cells Transformed in vitro Spontaneously and After Treatment with Nitrosomethylurea
title_full_unstemmed The Histological Appearance of Tumours Derived from Rat Embryo Cells Transformed in vitro Spontaneously and After Treatment with Nitrosomethylurea
title_short The Histological Appearance of Tumours Derived from Rat Embryo Cells Transformed in vitro Spontaneously and After Treatment with Nitrosomethylurea
title_sort histological appearance of tumours derived from rat embryo cells transformed in vitro spontaneously and after treatment with nitrosomethylurea
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008912/
https://www.ncbi.nlm.nih.gov/pubmed/4758371
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