Malignant Transformation in vitro of Rat Liver Cells by Dimethylnitrosamine and N-Methyl-N′-nitro-N-Nitrosoguanidine

Epithelial-like cells originating from 10-day and 8-week old BD rats were treated in vitro with dimethylnitrosamine (DMN) and N-methyl-N′-nitro-nitrosoguanidine (MNNG). Morphologically, no differences were seen between treated and untreated cells up to the time when the cells were transplanted into...

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Detalles Bibliográficos
Autores principales: Montesano, R., Vincent, L. Saint, Tomatis, L.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1973
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008984/
https://www.ncbi.nlm.nih.gov/pubmed/4743905
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author Montesano, R.
Vincent, L. Saint
Tomatis, L.
author_facet Montesano, R.
Vincent, L. Saint
Tomatis, L.
author_sort Montesano, R.
collection PubMed
description Epithelial-like cells originating from 10-day and 8-week old BD rats were treated in vitro with dimethylnitrosamine (DMN) and N-methyl-N′-nitro-nitrosoguanidine (MNNG). Morphologically, no differences were seen between treated and untreated cells up to the time when the cells were transplanted into syngeneic hosts. However, the treated cells grew in soft agar and once injected subcutaneously or intraperitoneally into newborn rats, developed tumours after a latent period of 9-12 weeks. The tumours obtained with DMN-treated cells were well differentiated adenocarcinomata, whereas carcinosarcomata were observed with the MNNG-treated cells. IMAGES:
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spelling pubmed-20089842009-09-10 Malignant Transformation in vitro of Rat Liver Cells by Dimethylnitrosamine and N-Methyl-N′-nitro-N-Nitrosoguanidine Montesano, R. Vincent, L. Saint Tomatis, L. Br J Cancer Articles Epithelial-like cells originating from 10-day and 8-week old BD rats were treated in vitro with dimethylnitrosamine (DMN) and N-methyl-N′-nitro-nitrosoguanidine (MNNG). Morphologically, no differences were seen between treated and untreated cells up to the time when the cells were transplanted into syngeneic hosts. However, the treated cells grew in soft agar and once injected subcutaneously or intraperitoneally into newborn rats, developed tumours after a latent period of 9-12 weeks. The tumours obtained with DMN-treated cells were well differentiated adenocarcinomata, whereas carcinosarcomata were observed with the MNNG-treated cells. IMAGES: Nature Publishing Group 1973-09 /pmc/articles/PMC2008984/ /pubmed/4743905 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Montesano, R.
Vincent, L. Saint
Tomatis, L.
Malignant Transformation in vitro of Rat Liver Cells by Dimethylnitrosamine and N-Methyl-N′-nitro-N-Nitrosoguanidine
title Malignant Transformation in vitro of Rat Liver Cells by Dimethylnitrosamine and N-Methyl-N′-nitro-N-Nitrosoguanidine
title_full Malignant Transformation in vitro of Rat Liver Cells by Dimethylnitrosamine and N-Methyl-N′-nitro-N-Nitrosoguanidine
title_fullStr Malignant Transformation in vitro of Rat Liver Cells by Dimethylnitrosamine and N-Methyl-N′-nitro-N-Nitrosoguanidine
title_full_unstemmed Malignant Transformation in vitro of Rat Liver Cells by Dimethylnitrosamine and N-Methyl-N′-nitro-N-Nitrosoguanidine
title_short Malignant Transformation in vitro of Rat Liver Cells by Dimethylnitrosamine and N-Methyl-N′-nitro-N-Nitrosoguanidine
title_sort malignant transformation in vitro of rat liver cells by dimethylnitrosamine and n-methyl-n′-nitro-n-nitrosoguanidine
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008984/
https://www.ncbi.nlm.nih.gov/pubmed/4743905
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