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Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle

Bleomycin inhibits cell division in HeLa S-3 and other mammalian epithelial cell lines. Its effect on suspension cultured cells is far greater than on monolayer cells; this was found to be due partly to the absence of divalent ions. Other mammalian epithelial lines in suspension culture are very sen...

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Detalles Bibliográficos
Autores principales: Wheatley, D. N., Mueller, G. C., Kajiwara, K.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1974
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008994/
https://www.ncbi.nlm.nih.gov/pubmed/4133781
Descripción
Sumario:Bleomycin inhibits cell division in HeLa S-3 and other mammalian epithelial cell lines. Its effect on suspension cultured cells is far greater than on monolayer cells; this was found to be due partly to the absence of divalent ions. Other mammalian epithelial lines in suspension culture are very sensitive but fibroblastic cells (BHK 21/C13/DWS-3) grown under identical conditions are relatively insensitive. This study has established that bleomycin is a powerful anti-mitotic agent during the G(2) phase but it does not prevent prophase cells from completing a normal mitotic division. Its ability to suppress DNA synthesis contributes little to the inhibition of cell cycling.