Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle

Bleomycin inhibits cell division in HeLa S-3 and other mammalian epithelial cell lines. Its effect on suspension cultured cells is far greater than on monolayer cells; this was found to be due partly to the absence of divalent ions. Other mammalian epithelial lines in suspension culture are very sen...

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Detalles Bibliográficos
Autores principales: Wheatley, D. N., Mueller, G. C., Kajiwara, K.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1974
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008994/
https://www.ncbi.nlm.nih.gov/pubmed/4133781
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author Wheatley, D. N.
Mueller, G. C.
Kajiwara, K.
author_facet Wheatley, D. N.
Mueller, G. C.
Kajiwara, K.
author_sort Wheatley, D. N.
collection PubMed
description Bleomycin inhibits cell division in HeLa S-3 and other mammalian epithelial cell lines. Its effect on suspension cultured cells is far greater than on monolayer cells; this was found to be due partly to the absence of divalent ions. Other mammalian epithelial lines in suspension culture are very sensitive but fibroblastic cells (BHK 21/C13/DWS-3) grown under identical conditions are relatively insensitive. This study has established that bleomycin is a powerful anti-mitotic agent during the G(2) phase but it does not prevent prophase cells from completing a normal mitotic division. Its ability to suppress DNA synthesis contributes little to the inhibition of cell cycling.
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spelling pubmed-20089942009-09-10 Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle Wheatley, D. N. Mueller, G. C. Kajiwara, K. Br J Cancer Articles Bleomycin inhibits cell division in HeLa S-3 and other mammalian epithelial cell lines. Its effect on suspension cultured cells is far greater than on monolayer cells; this was found to be due partly to the absence of divalent ions. Other mammalian epithelial lines in suspension culture are very sensitive but fibroblastic cells (BHK 21/C13/DWS-3) grown under identical conditions are relatively insensitive. This study has established that bleomycin is a powerful anti-mitotic agent during the G(2) phase but it does not prevent prophase cells from completing a normal mitotic division. Its ability to suppress DNA synthesis contributes little to the inhibition of cell cycling. Nature Publishing Group 1974-02 /pmc/articles/PMC2008994/ /pubmed/4133781 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Wheatley, D. N.
Mueller, G. C.
Kajiwara, K.
Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle
title Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle
title_full Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle
title_fullStr Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle
title_full_unstemmed Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle
title_short Anti-mitotic Activity of Bleomycin: Time of Action in the Mammalian Cell Cycle
title_sort anti-mitotic activity of bleomycin: time of action in the mammalian cell cycle
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2008994/
https://www.ncbi.nlm.nih.gov/pubmed/4133781
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AT kajiwarak antimitoticactivityofbleomycintimeofactioninthemammaliancellcycle

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