Inhibitors of Haemopoietic Cell Proliferation?: Specificity of Action Within the Haemopoietic System

The specificity of action of mature blood cell extracts on their own progenitor cells was investigated by measuring their effects on the structuredness of the cytoplasmic matrix (SCM) using the technique of fluorescence polarization. Changes in SCM induced by the various extracts are probably closel...

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Autores principales: Lord, B. I., Cercek, L., Cercek, B., Shah, G. P., Dexter, T. M., Lajtha, L. G.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1974
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009005/
https://www.ncbi.nlm.nih.gov/pubmed/4857338
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author Lord, B. I.
Cercek, L.
Cercek, B.
Shah, G. P.
Dexter, T. M.
Lajtha, L. G.
author_facet Lord, B. I.
Cercek, L.
Cercek, B.
Shah, G. P.
Dexter, T. M.
Lajtha, L. G.
author_sort Lord, B. I.
collection PubMed
description The specificity of action of mature blood cell extracts on their own progenitor cells was investigated by measuring their effects on the structuredness of the cytoplasmic matrix (SCM) using the technique of fluorescence polarization. Changes in SCM induced by the various extracts are probably closely related to the proliferation state of the cells. Saline extracts of lymphocytes, granulocytes and erythrocytes (LNE, GCE and RCE respectively) have been partially purified by ultrafiltration into selected molecular weight ranges and each tested against proliferative populations of lymphoid, granulocytic and erythroid cells. In all cases, complete specificity of effect on SCM was found, LNEs affecting only lymphoid cell populations, GCEs affecting only the granulocytic cell populations and RCEs affecting only erythroid cells. In each case, with the possible exception of the RCEs, the active fractions reside in the molecular weight ranges reported in the literature for cell extracts possessing proliferation inhibitory properties.
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spelling pubmed-20090052009-09-10 Inhibitors of Haemopoietic Cell Proliferation?: Specificity of Action Within the Haemopoietic System Lord, B. I. Cercek, L. Cercek, B. Shah, G. P. Dexter, T. M. Lajtha, L. G. Br J Cancer Articles The specificity of action of mature blood cell extracts on their own progenitor cells was investigated by measuring their effects on the structuredness of the cytoplasmic matrix (SCM) using the technique of fluorescence polarization. Changes in SCM induced by the various extracts are probably closely related to the proliferation state of the cells. Saline extracts of lymphocytes, granulocytes and erythrocytes (LNE, GCE and RCE respectively) have been partially purified by ultrafiltration into selected molecular weight ranges and each tested against proliferative populations of lymphoid, granulocytic and erythroid cells. In all cases, complete specificity of effect on SCM was found, LNEs affecting only lymphoid cell populations, GCEs affecting only the granulocytic cell populations and RCEs affecting only erythroid cells. In each case, with the possible exception of the RCEs, the active fractions reside in the molecular weight ranges reported in the literature for cell extracts possessing proliferation inhibitory properties. Nature Publishing Group 1974-02 /pmc/articles/PMC2009005/ /pubmed/4857338 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Lord, B. I.
Cercek, L.
Cercek, B.
Shah, G. P.
Dexter, T. M.
Lajtha, L. G.
Inhibitors of Haemopoietic Cell Proliferation?: Specificity of Action Within the Haemopoietic System
title Inhibitors of Haemopoietic Cell Proliferation?: Specificity of Action Within the Haemopoietic System
title_full Inhibitors of Haemopoietic Cell Proliferation?: Specificity of Action Within the Haemopoietic System
title_fullStr Inhibitors of Haemopoietic Cell Proliferation?: Specificity of Action Within the Haemopoietic System
title_full_unstemmed Inhibitors of Haemopoietic Cell Proliferation?: Specificity of Action Within the Haemopoietic System
title_short Inhibitors of Haemopoietic Cell Proliferation?: Specificity of Action Within the Haemopoietic System
title_sort inhibitors of haemopoietic cell proliferation?: specificity of action within the haemopoietic system
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009005/
https://www.ncbi.nlm.nih.gov/pubmed/4857338
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