Differences in the Urea-extracted Proteins of Mouse Epidermis and Squamous-cell Carcinomata Determined by Fluorescence Microscopy

Fluorescence microscopy was used to demonstrate differences in the urea-extractable antigens of mouse epidermis and squamous cell carcinoma. When serum- and normal tissue sediment-absorbed antisera prepared against mouse epidermal urea-extracted proteins were further absorbed with carcinoma urea ant...

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Autor principal: Carruthers, C.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1973
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009041/
https://www.ncbi.nlm.nih.gov/pubmed/4198974
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author Carruthers, C.
author_facet Carruthers, C.
author_sort Carruthers, C.
collection PubMed
description Fluorescence microscopy was used to demonstrate differences in the urea-extractable antigens of mouse epidermis and squamous cell carcinoma. When serum- and normal tissue sediment-absorbed antisera prepared against mouse epidermal urea-extracted proteins were further absorbed with carcinoma urea antigens, antisera specific for epidermis resulted. When antisera raised against the urea-extractable proteins of mouse squamous cell carcinomata were serum- and normal tissue sediment-absorbed and then further absorbed with epidermal urea antigens, antisera were prepared which stained papilloma and carcinoma, but not epidermis, and thus these antisera were not specific for carcinoma. Antisera prepared against the urea-extractable proteins of human epidermis reacted in immunodiffusion in agar with the epidermal urea proteins, but not with human squamous cell carcinoma urea proteins. Also antisera prepared against the carcinoma urea-extractable proteins reacted with these proteins in agar, but no reaction occurred with the epidermal urea-extractable proteins. IMAGES:
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spelling pubmed-20090412009-09-10 Differences in the Urea-extracted Proteins of Mouse Epidermis and Squamous-cell Carcinomata Determined by Fluorescence Microscopy Carruthers, C. Br J Cancer Articles Fluorescence microscopy was used to demonstrate differences in the urea-extractable antigens of mouse epidermis and squamous cell carcinoma. When serum- and normal tissue sediment-absorbed antisera prepared against mouse epidermal urea-extracted proteins were further absorbed with carcinoma urea antigens, antisera specific for epidermis resulted. When antisera raised against the urea-extractable proteins of mouse squamous cell carcinomata were serum- and normal tissue sediment-absorbed and then further absorbed with epidermal urea antigens, antisera were prepared which stained papilloma and carcinoma, but not epidermis, and thus these antisera were not specific for carcinoma. Antisera prepared against the urea-extractable proteins of human epidermis reacted in immunodiffusion in agar with the epidermal urea proteins, but not with human squamous cell carcinoma urea proteins. Also antisera prepared against the carcinoma urea-extractable proteins reacted with these proteins in agar, but no reaction occurred with the epidermal urea-extractable proteins. IMAGES: Nature Publishing Group 1973-07 /pmc/articles/PMC2009041/ /pubmed/4198974 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Carruthers, C.
Differences in the Urea-extracted Proteins of Mouse Epidermis and Squamous-cell Carcinomata Determined by Fluorescence Microscopy
title Differences in the Urea-extracted Proteins of Mouse Epidermis and Squamous-cell Carcinomata Determined by Fluorescence Microscopy
title_full Differences in the Urea-extracted Proteins of Mouse Epidermis and Squamous-cell Carcinomata Determined by Fluorescence Microscopy
title_fullStr Differences in the Urea-extracted Proteins of Mouse Epidermis and Squamous-cell Carcinomata Determined by Fluorescence Microscopy
title_full_unstemmed Differences in the Urea-extracted Proteins of Mouse Epidermis and Squamous-cell Carcinomata Determined by Fluorescence Microscopy
title_short Differences in the Urea-extracted Proteins of Mouse Epidermis and Squamous-cell Carcinomata Determined by Fluorescence Microscopy
title_sort differences in the urea-extracted proteins of mouse epidermis and squamous-cell carcinomata determined by fluorescence microscopy
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009041/
https://www.ncbi.nlm.nih.gov/pubmed/4198974
work_keys_str_mv AT carruthersc differencesintheureaextractedproteinsofmouseepidermisandsquamouscellcarcinomatadeterminedbyfluorescencemicroscopy

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