Human Peripheral Lymphocytes and Cancer: In Vitro Studies on the Basic Protein, Histone F2A1 Fraction

The interaction of the highly purified basic protein “antigen”, calf thymus histone F2A1 fraction, with peripheral lymphocytes isolated from patients with cancer and from normal subjects has been studied. Analysis, by SDS polyacrylamide gel electrophoresis of the basic protein remaining in the super...

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Detalles Bibliográficos
Autores principales: Fish, R. G., Pritchard, J. A. V., Deeley, T. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1974
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009212/
https://www.ncbi.nlm.nih.gov/pubmed/4476219
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author Fish, R. G.
Pritchard, J. A. V.
Deeley, T. J.
author_facet Fish, R. G.
Pritchard, J. A. V.
Deeley, T. J.
author_sort Fish, R. G.
collection PubMed
description The interaction of the highly purified basic protein “antigen”, calf thymus histone F2A1 fraction, with peripheral lymphocytes isolated from patients with cancer and from normal subjects has been studied. Analysis, by SDS polyacrylamide gel electrophoresis of the basic protein remaining in the supernatant fluid after interaction with low concentrations of lymphocytes from patients showed the presence of a component(s) of molecular weight smaller than the original histone F2A1 fraction. Similar experiments using lymphocytes derived from normal subjects indicated that this component(s) is absent, or at least is present in only small amounts. This difference could be partially abolished by using high concentrations of cell preparations. It is suggested that the observed difference is due at least in part, to differences in protease activity between the two preparations. The possible significance of these findings in relation to the macrophage electrophoretic mobility test for cancer is discussed. IMAGES:
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spelling pubmed-20092122009-09-10 Human Peripheral Lymphocytes and Cancer: In Vitro Studies on the Basic Protein, Histone F2A1 Fraction Fish, R. G. Pritchard, J. A. V. Deeley, T. J. Br J Cancer Articles The interaction of the highly purified basic protein “antigen”, calf thymus histone F2A1 fraction, with peripheral lymphocytes isolated from patients with cancer and from normal subjects has been studied. Analysis, by SDS polyacrylamide gel electrophoresis of the basic protein remaining in the supernatant fluid after interaction with low concentrations of lymphocytes from patients showed the presence of a component(s) of molecular weight smaller than the original histone F2A1 fraction. Similar experiments using lymphocytes derived from normal subjects indicated that this component(s) is absent, or at least is present in only small amounts. This difference could be partially abolished by using high concentrations of cell preparations. It is suggested that the observed difference is due at least in part, to differences in protease activity between the two preparations. The possible significance of these findings in relation to the macrophage electrophoretic mobility test for cancer is discussed. IMAGES: Nature Publishing Group 1974-09 /pmc/articles/PMC2009212/ /pubmed/4476219 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Fish, R. G.
Pritchard, J. A. V.
Deeley, T. J.
Human Peripheral Lymphocytes and Cancer: In Vitro Studies on the Basic Protein, Histone F2A1 Fraction
title Human Peripheral Lymphocytes and Cancer: In Vitro Studies on the Basic Protein, Histone F2A1 Fraction
title_full Human Peripheral Lymphocytes and Cancer: In Vitro Studies on the Basic Protein, Histone F2A1 Fraction
title_fullStr Human Peripheral Lymphocytes and Cancer: In Vitro Studies on the Basic Protein, Histone F2A1 Fraction
title_full_unstemmed Human Peripheral Lymphocytes and Cancer: In Vitro Studies on the Basic Protein, Histone F2A1 Fraction
title_short Human Peripheral Lymphocytes and Cancer: In Vitro Studies on the Basic Protein, Histone F2A1 Fraction
title_sort human peripheral lymphocytes and cancer: in vitro studies on the basic protein, histone f2a1 fraction
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009212/
https://www.ncbi.nlm.nih.gov/pubmed/4476219
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