A Tumour Cell Aggregation Promoting Substance from Rat Ascites Hepatoma Cells*

A substance capable of promoting tumour cell aggregation was released from rat ascites hepatoma cell (possibly from the cell surface) kept in Hanks' balanced salt solution (free of calcium and magnesium) in the cold, and then partially purified by chromatography with DEAE-Sephadex and gel filtr...

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Detalles Bibliográficos
Autores principales: Kudo, K., Tasaki, I., Hanaoka, Y., Hayashi, H.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1974
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009342/
https://www.ncbi.nlm.nih.gov/pubmed/4374963
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author Kudo, K.
Tasaki, I.
Hanaoka, Y.
Hayashi, H.
author_facet Kudo, K.
Tasaki, I.
Hanaoka, Y.
Hayashi, H.
author_sort Kudo, K.
collection PubMed
description A substance capable of promoting tumour cell aggregation was released from rat ascites hepatoma cell (possibly from the cell surface) kept in Hanks' balanced salt solution (free of calcium and magnesium) in the cold, and then partially purified by chromatography with DEAE-Sephadex and gel filtration with Bio-gel. The thermostable substance seemed to be a glycoprotein and its molecular weight was about 72,000 when measured by gel filtration on Sephadex G-200. It had no proteolytic activity. The material was clearly effective for rat ascites hepatoma cells as well as SV40 transformed cells, but less effective for Chang's cells and apparently ineffective for normal rat liver cells and red blood cells. The action of this material was more potent than that of Jack bean concanavalin A when assayed for aggregation of SV40 transformed cells. Its effect was not influenced by concanavalin A inhibitors such as alpha-methyl-D-glucopyranoside, N-acetyl-D-glucosamine and D-glucose. IMAGES:
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spelling pubmed-20093422009-09-10 A Tumour Cell Aggregation Promoting Substance from Rat Ascites Hepatoma Cells* Kudo, K. Tasaki, I. Hanaoka, Y. Hayashi, H. Br J Cancer Articles A substance capable of promoting tumour cell aggregation was released from rat ascites hepatoma cell (possibly from the cell surface) kept in Hanks' balanced salt solution (free of calcium and magnesium) in the cold, and then partially purified by chromatography with DEAE-Sephadex and gel filtration with Bio-gel. The thermostable substance seemed to be a glycoprotein and its molecular weight was about 72,000 when measured by gel filtration on Sephadex G-200. It had no proteolytic activity. The material was clearly effective for rat ascites hepatoma cells as well as SV40 transformed cells, but less effective for Chang's cells and apparently ineffective for normal rat liver cells and red blood cells. The action of this material was more potent than that of Jack bean concanavalin A when assayed for aggregation of SV40 transformed cells. Its effect was not influenced by concanavalin A inhibitors such as alpha-methyl-D-glucopyranoside, N-acetyl-D-glucosamine and D-glucose. IMAGES: Nature Publishing Group 1974-12 /pmc/articles/PMC2009342/ /pubmed/4374963 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Articles
Kudo, K.
Tasaki, I.
Hanaoka, Y.
Hayashi, H.
A Tumour Cell Aggregation Promoting Substance from Rat Ascites Hepatoma Cells*
title A Tumour Cell Aggregation Promoting Substance from Rat Ascites Hepatoma Cells*
title_full A Tumour Cell Aggregation Promoting Substance from Rat Ascites Hepatoma Cells*
title_fullStr A Tumour Cell Aggregation Promoting Substance from Rat Ascites Hepatoma Cells*
title_full_unstemmed A Tumour Cell Aggregation Promoting Substance from Rat Ascites Hepatoma Cells*
title_short A Tumour Cell Aggregation Promoting Substance from Rat Ascites Hepatoma Cells*
title_sort tumour cell aggregation promoting substance from rat ascites hepatoma cells*
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009342/
https://www.ncbi.nlm.nih.gov/pubmed/4374963
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