Biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells.

Various antigenic activities of polyoma virus-transformed and embryonic mouse cells were retained after 3M KCl solubilization of surface components. Particularly, transplantation antigen (TSTA) demonstrated by homograft rejection, and surface (S) antigen, detected by inhibition of immunofluorescence...

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Autores principales: Kitahara, Y., Barra, Y., Meyer, G.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009490/
https://www.ncbi.nlm.nih.gov/pubmed/202299
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author Kitahara, Y.
Barra, Y.
Meyer, G.
author_facet Kitahara, Y.
Barra, Y.
Meyer, G.
author_sort Kitahara, Y.
collection PubMed
description Various antigenic activities of polyoma virus-transformed and embryonic mouse cells were retained after 3M KCl solubilization of surface components. Particularly, transplantation antigen (TSTA) demonstrated by homograft rejection, and surface (S) antigen, detected by inhibition of immunofluorescence on polyoma-virus-transformed mouse cells, could be demonstrated. The crude soluble extracts were partially purified by salting out with (NH4)2SO4. In the case of polyoma-virus-transformed cells, TSTA and a part of S antigen activity were found in the same fraction (60% (NH4)2SO4 saturation) while another part of S antigen was salted out at 80% saturation. By chromatography, S antigen activity was found in 2 zones for transformed cells and in one zone for embryonic cells. One of these zones was common to both cell extracts.
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spelling pubmed-20094902009-09-10 Biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells. Kitahara, Y. Barra, Y. Meyer, G. Br J Cancer Research Article Various antigenic activities of polyoma virus-transformed and embryonic mouse cells were retained after 3M KCl solubilization of surface components. Particularly, transplantation antigen (TSTA) demonstrated by homograft rejection, and surface (S) antigen, detected by inhibition of immunofluorescence on polyoma-virus-transformed mouse cells, could be demonstrated. The crude soluble extracts were partially purified by salting out with (NH4)2SO4. In the case of polyoma-virus-transformed cells, TSTA and a part of S antigen activity were found in the same fraction (60% (NH4)2SO4 saturation) while another part of S antigen was salted out at 80% saturation. By chromatography, S antigen activity was found in 2 zones for transformed cells and in one zone for embryonic cells. One of these zones was common to both cell extracts. Nature Publishing Group 1978-01 /pmc/articles/PMC2009490/ /pubmed/202299 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Kitahara, Y.
Barra, Y.
Meyer, G.
Biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells.
title Biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells.
title_full Biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells.
title_fullStr Biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells.
title_full_unstemmed Biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells.
title_short Biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells.
title_sort biological activities of solubilized surface antigens of embryonic and polyoma-virus-transformed cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009490/
https://www.ncbi.nlm.nih.gov/pubmed/202299
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