Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry.

The reaction rates of enzymes hydrolysing fluorescein diacetate have been studied in populations of intact tissue-culture EMT6, cells using flow cytofluorimetric techniques. It was found that the activity of these enzymes increased in plateau phases and that this correlated inversely with plating ef...

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Detalles Bibliográficos
Autores principales: Watson, J. V., Workman, P., Chambers, S. H.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009517/
https://www.ncbi.nlm.nih.gov/pubmed/638017
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author Watson, J. V.
Workman, P.
Chambers, S. H.
author_facet Watson, J. V.
Workman, P.
Chambers, S. H.
author_sort Watson, J. V.
collection PubMed
description The reaction rates of enzymes hydrolysing fluorescein diacetate have been studied in populations of intact tissue-culture EMT6, cells using flow cytofluorimetric techniques. It was found that the activity of these enzymes increased in plateau phases and that this correlated inversely with plating efficiency. Highly abnormal substrate-dependent reaction velocity kinetics were found in 14-, 21-, 28- and 35-day cultures.
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spelling pubmed-20095172009-09-10 Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry. Watson, J. V. Workman, P. Chambers, S. H. Br J Cancer Research Article The reaction rates of enzymes hydrolysing fluorescein diacetate have been studied in populations of intact tissue-culture EMT6, cells using flow cytofluorimetric techniques. It was found that the activity of these enzymes increased in plateau phases and that this correlated inversely with plating efficiency. Highly abnormal substrate-dependent reaction velocity kinetics were found in 14-, 21-, 28- and 35-day cultures. Nature Publishing Group 1978-03 /pmc/articles/PMC2009517/ /pubmed/638017 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Watson, J. V.
Workman, P.
Chambers, S. H.
Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry.
title Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry.
title_full Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry.
title_fullStr Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry.
title_full_unstemmed Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry.
title_short Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry.
title_sort different esterase activities of exponential and plateau phases of emt6 cells monitored by flow cytofluorimetry.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009517/
https://www.ncbi.nlm.nih.gov/pubmed/638017
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AT workmanp differentesteraseactivitiesofexponentialandplateauphasesofemt6cellsmonitoredbyflowcytofluorimetry
AT chamberssh differentesteraseactivitiesofexponentialandplateauphasesofemt6cellsmonitoredbyflowcytofluorimetry

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