Ultrastructure and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro.

The ultrastructure and in vitro growth properties of 5 tumorigenic mouse submandibular-gland epithelial cell lines were studied. In all lines, in vitro acinus formation occurred, and well differentiated epithelial cells showing epithelial microvilli and desmosomes and cytoplasmic tonofilaments were...

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Detalles Bibliográficos
Autores principales: Knowles, M. A., Franks, L. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009545/
https://www.ncbi.nlm.nih.gov/pubmed/646931
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author Knowles, M. A.
Franks, L. M.
author_facet Knowles, M. A.
Franks, L. M.
author_sort Knowles, M. A.
collection PubMed
description The ultrastructure and in vitro growth properties of 5 tumorigenic mouse submandibular-gland epithelial cell lines were studied. In all lines, in vitro acinus formation occurred, and well differentiated epithelial cells showing epithelial microvilli and desmosomes and cytoplasmic tonofilaments were present. None of the cells showed specific ultratructural features of the normal differentiated submandibular-gland ducts. All the lines formed colonies in semi-solid agar and on confluent monolayers of BALB/c 3T3 cells, and all lacked density-dependent inhibition of growth, as demonstrated by a random distribution of [3H]TdR labelling throughout growing colonies. These 3 growth properties appear to be reliable in vitro markers for epithelial neoplastic transformation in this system, but colony-forming efficiency in agar is lower than that reported for many transformed mesenchymal cells. IMAGES:
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spelling pubmed-20095452009-09-10 Ultrastructure and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro. Knowles, M. A. Franks, L. M. Br J Cancer Research Article The ultrastructure and in vitro growth properties of 5 tumorigenic mouse submandibular-gland epithelial cell lines were studied. In all lines, in vitro acinus formation occurred, and well differentiated epithelial cells showing epithelial microvilli and desmosomes and cytoplasmic tonofilaments were present. None of the cells showed specific ultratructural features of the normal differentiated submandibular-gland ducts. All the lines formed colonies in semi-solid agar and on confluent monolayers of BALB/c 3T3 cells, and all lacked density-dependent inhibition of growth, as demonstrated by a random distribution of [3H]TdR labelling throughout growing colonies. These 3 growth properties appear to be reliable in vitro markers for epithelial neoplastic transformation in this system, but colony-forming efficiency in agar is lower than that reported for many transformed mesenchymal cells. IMAGES: Nature Publishing Group 1978-04 /pmc/articles/PMC2009545/ /pubmed/646931 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Knowles, M. A.
Franks, L. M.
Ultrastructure and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro.
title Ultrastructure and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro.
title_full Ultrastructure and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro.
title_fullStr Ultrastructure and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro.
title_full_unstemmed Ultrastructure and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro.
title_short Ultrastructure and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro.
title_sort ultrastructure and biological markers of neoplastic change in adult mouse epithelial cells transformed in vitro.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009545/
https://www.ncbi.nlm.nih.gov/pubmed/646931
work_keys_str_mv AT knowlesma ultrastructureandbiologicalmarkersofneoplasticchangeinadultmouseepithelialcellstransformedinvitro
AT frankslm ultrastructureandbiologicalmarkersofneoplasticchangeinadultmouseepithelialcellstransformedinvitro

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