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An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents.
An in vitro agar colony technique has been developed for the growth of tumour cells taken directly from human tumours grown in immune-suppressed mice. The novel feature of the technique is the addition of a replenishable liquid phase which permits the maintenance of relatively slowly growing cells....
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1978
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009601/ https://www.ncbi.nlm.nih.gov/pubmed/343804 |
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author | Courtenay, V. D. Mills, J. |
author_facet | Courtenay, V. D. Mills, J. |
author_sort | Courtenay, V. D. |
collection | PubMed |
description | An in vitro agar colony technique has been developed for the growth of tumour cells taken directly from human tumours grown in immune-suppressed mice. The novel feature of the technique is the addition of a replenishable liquid phase which permits the maintenance of relatively slowly growing cells. A number of different xenografted tumours have been cultured successfully in this system, with red blood cells added to the agar and using 5% O2 in the gas phase. The technique has been used to assay cell survival in tumours treated in vivo with cytotoxic agents, and examples are given of survival curves obtained from a pancreatic tumours irradiated with gamma-rays and a colonic tumour from mice treated with cyclophosphamide. The results obtained by this in vitro method are in agreement with those from the agar diffusion chamber technique. This culture method has also been successfully used for the growth of cells taken directly from human tumour biopsy samples obtained in the clinic. IMAGES: |
format | Text |
id | pubmed-2009601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1978 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20096012009-09-10 An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. Courtenay, V. D. Mills, J. Br J Cancer Research Article An in vitro agar colony technique has been developed for the growth of tumour cells taken directly from human tumours grown in immune-suppressed mice. The novel feature of the technique is the addition of a replenishable liquid phase which permits the maintenance of relatively slowly growing cells. A number of different xenografted tumours have been cultured successfully in this system, with red blood cells added to the agar and using 5% O2 in the gas phase. The technique has been used to assay cell survival in tumours treated in vivo with cytotoxic agents, and examples are given of survival curves obtained from a pancreatic tumours irradiated with gamma-rays and a colonic tumour from mice treated with cyclophosphamide. The results obtained by this in vitro method are in agreement with those from the agar diffusion chamber technique. This culture method has also been successfully used for the growth of cells taken directly from human tumour biopsy samples obtained in the clinic. IMAGES: Nature Publishing Group 1978-02 /pmc/articles/PMC2009601/ /pubmed/343804 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Courtenay, V. D. Mills, J. An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. |
title | An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. |
title_full | An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. |
title_fullStr | An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. |
title_full_unstemmed | An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. |
title_short | An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. |
title_sort | in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009601/ https://www.ncbi.nlm.nih.gov/pubmed/343804 |
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