Stability of transplanted murine tumour systems after storage of cells at -196 degrees C for up to 13 years.

Two murine lymphomas of spontaneous origin were stored in liquid N2 as cell suspensions in DMSO for 8 and 13 years respectively. Isogenic transplantation assays done some years before freezing and immediately after thawing indicated no measurable loss of clonogenic cells during freezing, storage or...

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Detalles Bibliográficos
Autores principales: Hewitt, H. B., Blake, E. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009631/
https://www.ncbi.nlm.nih.gov/pubmed/656301
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author Hewitt, H. B.
Blake, E. R.
author_facet Hewitt, H. B.
Blake, E. R.
author_sort Hewitt, H. B.
collection PubMed
description Two murine lymphomas of spontaneous origin were stored in liquid N2 as cell suspensions in DMSO for 8 and 13 years respectively. Isogenic transplantation assays done some years before freezing and immediately after thawing indicated no measurable loss of clonogenic cells during freezing, storage or thawing; the number of cells required for 50% successful transplantation remained close to unity in both cases. The overall data revealed no evidence of an alteration of the receptivity of the mouse colonies over a period of 13 or 20 years. We attribute this remarkable stability to close supervision of the systems within a single laboratory.
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spelling pubmed-20096312009-09-10 Stability of transplanted murine tumour systems after storage of cells at -196 degrees C for up to 13 years. Hewitt, H. B. Blake, E. R. Br J Cancer Research Article Two murine lymphomas of spontaneous origin were stored in liquid N2 as cell suspensions in DMSO for 8 and 13 years respectively. Isogenic transplantation assays done some years before freezing and immediately after thawing indicated no measurable loss of clonogenic cells during freezing, storage or thawing; the number of cells required for 50% successful transplantation remained close to unity in both cases. The overall data revealed no evidence of an alteration of the receptivity of the mouse colonies over a period of 13 or 20 years. We attribute this remarkable stability to close supervision of the systems within a single laboratory. Nature Publishing Group 1978-05 /pmc/articles/PMC2009631/ /pubmed/656301 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Hewitt, H. B.
Blake, E. R.
Stability of transplanted murine tumour systems after storage of cells at -196 degrees C for up to 13 years.
title Stability of transplanted murine tumour systems after storage of cells at -196 degrees C for up to 13 years.
title_full Stability of transplanted murine tumour systems after storage of cells at -196 degrees C for up to 13 years.
title_fullStr Stability of transplanted murine tumour systems after storage of cells at -196 degrees C for up to 13 years.
title_full_unstemmed Stability of transplanted murine tumour systems after storage of cells at -196 degrees C for up to 13 years.
title_short Stability of transplanted murine tumour systems after storage of cells at -196 degrees C for up to 13 years.
title_sort stability of transplanted murine tumour systems after storage of cells at -196 degrees c for up to 13 years.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009631/
https://www.ncbi.nlm.nih.gov/pubmed/656301
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