Antigen shedding by human breast-cancer cells in vitro and in vivo.

Human breast cancer cells were adapted to chemically defined medium in order to recover naturally shed glycoproteins. Sephadex G-150 chromatography of these glycoproteins revealed 1 major and 2 minor peaks. When the cells were grown in the presence of 3H-leucine, the radioactive shed proteins had a...

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Detalles Bibliográficos
Autores principales: Nordquist, R. E., Anglin, J. H., Lerner, M. P.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009636/
https://www.ncbi.nlm.nih.gov/pubmed/656305
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author Nordquist, R. E.
Anglin, J. H.
Lerner, M. P.
author_facet Nordquist, R. E.
Anglin, J. H.
Lerner, M. P.
author_sort Nordquist, R. E.
collection PubMed
description Human breast cancer cells were adapted to chemically defined medium in order to recover naturally shed glycoproteins. Sephadex G-150 chromatography of these glycoproteins revealed 1 major and 2 minor peaks. When the cells were grown in the presence of 3H-leucine, the radioactive shed proteins had a Sephadex profile identical to the unlabelled shed glycoproteins. PAGE analysis of these proteins showed 5 major bands. Antigenically similar proteins were found in the serum of female nude mice bearing BOT-2 tumours, but not in controls.
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spelling pubmed-20096362009-09-10 Antigen shedding by human breast-cancer cells in vitro and in vivo. Nordquist, R. E. Anglin, J. H. Lerner, M. P. Br J Cancer Research Article Human breast cancer cells were adapted to chemically defined medium in order to recover naturally shed glycoproteins. Sephadex G-150 chromatography of these glycoproteins revealed 1 major and 2 minor peaks. When the cells were grown in the presence of 3H-leucine, the radioactive shed proteins had a Sephadex profile identical to the unlabelled shed glycoproteins. PAGE analysis of these proteins showed 5 major bands. Antigenically similar proteins were found in the serum of female nude mice bearing BOT-2 tumours, but not in controls. Nature Publishing Group 1978-05 /pmc/articles/PMC2009636/ /pubmed/656305 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Nordquist, R. E.
Anglin, J. H.
Lerner, M. P.
Antigen shedding by human breast-cancer cells in vitro and in vivo.
title Antigen shedding by human breast-cancer cells in vitro and in vivo.
title_full Antigen shedding by human breast-cancer cells in vitro and in vivo.
title_fullStr Antigen shedding by human breast-cancer cells in vitro and in vivo.
title_full_unstemmed Antigen shedding by human breast-cancer cells in vitro and in vivo.
title_short Antigen shedding by human breast-cancer cells in vitro and in vivo.
title_sort antigen shedding by human breast-cancer cells in vitro and in vivo.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009636/
https://www.ncbi.nlm.nih.gov/pubmed/656305
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AT lernermp antigensheddingbyhumanbreastcancercellsinvitroandinvivo

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