A soft-agar procedure measuring growth of human colonic carcinomas.

Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens we...

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Detalles Bibliográficos
Autores principales: Kimball, P. M., Brattain, M. G., Pitts, A. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009639/
https://www.ncbi.nlm.nih.gov/pubmed/678422
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author Kimball, P. M.
Brattain, M. G.
Pitts, A. M.
author_facet Kimball, P. M.
Brattain, M. G.
Pitts, A. M.
author_sort Kimball, P. M.
collection PubMed
description Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens were 1.0-4.5%. The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens. Clusters were apparent after 3 weeks in culture, and maximum cluster formation was typically seen by 5 weeks. Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth. The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%. IMAGES:
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spelling pubmed-20096392009-09-10 A soft-agar procedure measuring growth of human colonic carcinomas. Kimball, P. M. Brattain, M. G. Pitts, A. M. Br J Cancer Research Article Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens were 1.0-4.5%. The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens. Clusters were apparent after 3 weeks in culture, and maximum cluster formation was typically seen by 5 weeks. Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth. The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%. IMAGES: Nature Publishing Group 1978-06 /pmc/articles/PMC2009639/ /pubmed/678422 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Kimball, P. M.
Brattain, M. G.
Pitts, A. M.
A soft-agar procedure measuring growth of human colonic carcinomas.
title A soft-agar procedure measuring growth of human colonic carcinomas.
title_full A soft-agar procedure measuring growth of human colonic carcinomas.
title_fullStr A soft-agar procedure measuring growth of human colonic carcinomas.
title_full_unstemmed A soft-agar procedure measuring growth of human colonic carcinomas.
title_short A soft-agar procedure measuring growth of human colonic carcinomas.
title_sort soft-agar procedure measuring growth of human colonic carcinomas.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009639/
https://www.ncbi.nlm.nih.gov/pubmed/678422
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