Growth of human tumour cell colonies from biopsies using two soft-agar techniques.

Two techniques for growing colonies of human tumour cells in soft agar have been applied to cell suspensions derived from fresh tumour tissue from 48 patients. Colonies were obtained in 31 cases, with plating efficiencies between 0.01 and 15%. In 11 cases the plating efficiencies were 1% or above. T...

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Autores principales: Courtenay, V. D., Selby, P. J., Smith, I. E., Mills, J., Peckham, M. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009691/
https://www.ncbi.nlm.nih.gov/pubmed/687520
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author Courtenay, V. D.
Selby, P. J.
Smith, I. E.
Mills, J.
Peckham, M. J.
author_facet Courtenay, V. D.
Selby, P. J.
Smith, I. E.
Mills, J.
Peckham, M. J.
author_sort Courtenay, V. D.
collection PubMed
description Two techniques for growing colonies of human tumour cells in soft agar have been applied to cell suspensions derived from fresh tumour tissue from 48 patients. Colonies were obtained in 31 cases, with plating efficiencies between 0.01 and 15%. In 11 cases the plating efficiencies were 1% or above. There was evidence that some categories of tumour grew more readily than others under these conditions. The potential applications of the methods to clinical and experimental oncology are discussed. IMAGES:
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spelling pubmed-20096912009-09-10 Growth of human tumour cell colonies from biopsies using two soft-agar techniques. Courtenay, V. D. Selby, P. J. Smith, I. E. Mills, J. Peckham, M. J. Br J Cancer Research Article Two techniques for growing colonies of human tumour cells in soft agar have been applied to cell suspensions derived from fresh tumour tissue from 48 patients. Colonies were obtained in 31 cases, with plating efficiencies between 0.01 and 15%. In 11 cases the plating efficiencies were 1% or above. There was evidence that some categories of tumour grew more readily than others under these conditions. The potential applications of the methods to clinical and experimental oncology are discussed. IMAGES: Nature Publishing Group 1978-07 /pmc/articles/PMC2009691/ /pubmed/687520 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Courtenay, V. D.
Selby, P. J.
Smith, I. E.
Mills, J.
Peckham, M. J.
Growth of human tumour cell colonies from biopsies using two soft-agar techniques.
title Growth of human tumour cell colonies from biopsies using two soft-agar techniques.
title_full Growth of human tumour cell colonies from biopsies using two soft-agar techniques.
title_fullStr Growth of human tumour cell colonies from biopsies using two soft-agar techniques.
title_full_unstemmed Growth of human tumour cell colonies from biopsies using two soft-agar techniques.
title_short Growth of human tumour cell colonies from biopsies using two soft-agar techniques.
title_sort growth of human tumour cell colonies from biopsies using two soft-agar techniques.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009691/
https://www.ncbi.nlm.nih.gov/pubmed/687520
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