Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma.

A cell line (J82) was derived from a poorly differentiated, invasive, transitional-cell carcinoma, Stage T3. The cells have been propagated in vitro for 5 years and showed 100% aneuploidy and a mixed epithelial-fibroblastic morphology. The majority of cells contained 2Y chromosomes and several disti...

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Autores principales: O'Toole, C., Price, Z. H., Ohnuki, Y., Unsgaard, B.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009694/
https://www.ncbi.nlm.nih.gov/pubmed/687519
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author O'Toole, C.
Price, Z. H.
Ohnuki, Y.
Unsgaard, B.
author_facet O'Toole, C.
Price, Z. H.
Ohnuki, Y.
Unsgaard, B.
author_sort O'Toole, C.
collection PubMed
description A cell line (J82) was derived from a poorly differentiated, invasive, transitional-cell carcinoma, Stage T3. The cells have been propagated in vitro for 5 years and showed 100% aneuploidy and a mixed epithelial-fibroblastic morphology. The majority of cells contained 2Y chromosomes and several distinctive markers. Peripheral-blood lymphocytes from the donor of the J82 cells were tested sequentially for cytotoxicity toward autologous and allogeneic tumour cells. Autologous cytotoxicity was detected against J82 cells in early in vitro passage. Allogeneic lymphocytes from some patients with transitional-cell carcinoma were also cytotoxic to J82 cells in primary culture. However, selective cytotoxicity by lymphoid cells from bladder-carcinoma patients was not detected against J82 cells in long-term tissue culture. IMAGES:
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spelling pubmed-20096942009-09-10 Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma. O'Toole, C. Price, Z. H. Ohnuki, Y. Unsgaard, B. Br J Cancer Research Article A cell line (J82) was derived from a poorly differentiated, invasive, transitional-cell carcinoma, Stage T3. The cells have been propagated in vitro for 5 years and showed 100% aneuploidy and a mixed epithelial-fibroblastic morphology. The majority of cells contained 2Y chromosomes and several distinctive markers. Peripheral-blood lymphocytes from the donor of the J82 cells were tested sequentially for cytotoxicity toward autologous and allogeneic tumour cells. Autologous cytotoxicity was detected against J82 cells in early in vitro passage. Allogeneic lymphocytes from some patients with transitional-cell carcinoma were also cytotoxic to J82 cells in primary culture. However, selective cytotoxicity by lymphoid cells from bladder-carcinoma patients was not detected against J82 cells in long-term tissue culture. IMAGES: Nature Publishing Group 1978-07 /pmc/articles/PMC2009694/ /pubmed/687519 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
O'Toole, C.
Price, Z. H.
Ohnuki, Y.
Unsgaard, B.
Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma.
title Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma.
title_full Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma.
title_fullStr Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma.
title_full_unstemmed Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma.
title_short Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma.
title_sort ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009694/
https://www.ncbi.nlm.nih.gov/pubmed/687519
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