E-rosette-forming cells at 29 degrees C: an assay for the evaluation of the immune status of cancer patients.

High-affinity rosette-forming T-cell assays were performed by incubation of peripheral-blood mononuclear cells with sheep erythrocytes (E) at 29 degrees C. As compared with normal controls, the levels of high-affinity rosette-forming cells (RFC) were much more frequently depressed in cancer patients...

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Detalles Bibliográficos
Autores principales: Quan, P. C., Burtin, P.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009783/
https://www.ncbi.nlm.nih.gov/pubmed/310311
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author Quan, P. C.
Burtin, P.
author_facet Quan, P. C.
Burtin, P.
author_sort Quan, P. C.
collection PubMed
description High-affinity rosette-forming T-cell assays were performed by incubation of peripheral-blood mononuclear cells with sheep erythrocytes (E) at 29 degrees C. As compared with normal controls, the levels of high-affinity rosette-forming cells (RFC) were much more frequently depressed in cancer patients than were the total E-RFC incubated at 4 degrees C. Only 2/83 normal controls had less than 38% 29 degrees C E-RFC (mean 48 +/- 5), whilst 78/89 cancer patients were below this level. The few postoperative patients tested exhibited a normal range of 29 degrees C E-RFC. The 29 degrees C E-rosette assay gives reproducible counts of a T-cell subset, and is a sensitive assay for evaluating the immune status of cancer patients.
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spelling pubmed-20097832009-09-10 E-rosette-forming cells at 29 degrees C: an assay for the evaluation of the immune status of cancer patients. Quan, P. C. Burtin, P. Br J Cancer Research Article High-affinity rosette-forming T-cell assays were performed by incubation of peripheral-blood mononuclear cells with sheep erythrocytes (E) at 29 degrees C. As compared with normal controls, the levels of high-affinity rosette-forming cells (RFC) were much more frequently depressed in cancer patients than were the total E-RFC incubated at 4 degrees C. Only 2/83 normal controls had less than 38% 29 degrees C E-RFC (mean 48 +/- 5), whilst 78/89 cancer patients were below this level. The few postoperative patients tested exhibited a normal range of 29 degrees C E-RFC. The 29 degrees C E-rosette assay gives reproducible counts of a T-cell subset, and is a sensitive assay for evaluating the immune status of cancer patients. Nature Publishing Group 1978-11 /pmc/articles/PMC2009783/ /pubmed/310311 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Quan, P. C.
Burtin, P.
E-rosette-forming cells at 29 degrees C: an assay for the evaluation of the immune status of cancer patients.
title E-rosette-forming cells at 29 degrees C: an assay for the evaluation of the immune status of cancer patients.
title_full E-rosette-forming cells at 29 degrees C: an assay for the evaluation of the immune status of cancer patients.
title_fullStr E-rosette-forming cells at 29 degrees C: an assay for the evaluation of the immune status of cancer patients.
title_full_unstemmed E-rosette-forming cells at 29 degrees C: an assay for the evaluation of the immune status of cancer patients.
title_short E-rosette-forming cells at 29 degrees C: an assay for the evaluation of the immune status of cancer patients.
title_sort e-rosette-forming cells at 29 degrees c: an assay for the evaluation of the immune status of cancer patients.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009783/
https://www.ncbi.nlm.nih.gov/pubmed/310311
work_keys_str_mv AT quanpc erosetteformingcellsat29degreescanassayfortheevaluationoftheimmunestatusofcancerpatients
AT burtinp erosetteformingcellsat29degreescanassayfortheevaluationoftheimmunestatusofcancerpatients

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