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Cell yield and cell survival following chemotherapy of the B16 melanoma.
We describe in this paper cell survival studies, using in vitro clonogenic assays, performed on the B16 melanoma treated in situ with various cytotoxic agents. In addition we have determined the effects of these agents on the yield of cells obtained by trypsinization. In untreated tumours the mean c...
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1978
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009784/ https://www.ncbi.nlm.nih.gov/pubmed/728348 |
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author | Stephens, T. C. Peacock, J. H. |
author_facet | Stephens, T. C. Peacock, J. H. |
author_sort | Stephens, T. C. |
collection | PubMed |
description | We describe in this paper cell survival studies, using in vitro clonogenic assays, performed on the B16 melanoma treated in situ with various cytotoxic agents. In addition we have determined the effects of these agents on the yield of cells obtained by trypsinization. In untreated tumours the mean cell yield was approximately 10(8)/g, which is 20--30% of the cells actually present in the tissue. The plating efficiency was approximately 40%. Most agents rapidly affected both cell yield and cell survival. For example, within 20--30 h, gamma-radiation and several alkylating agents reduced cell yield by about 40%. The cell yield change was associated with an increase in mean cell size. Cell yield was reduced even more (approximately 70%) by Vinca alkaloids. This large reduction was associated with extensive cell lysis, observed as an increase in the necrotic fraction of tumours from approximately 35% to approximately 70%. Adriamycin, bleomycin and Ara-C also produced a moderate reduction in cell yield (approximately 40%), but actinomycin D did not reduce cell yield and FU increased it by about 30%. Only gamma-radiation, cyclophosphamide, CCNU, BCNU and melphalan produced more than a 90% reduction in cell survival, although there was a small but measurable reduction with all other agents except vinblastine, HN2 and actinomycin D. |
format | Text |
id | pubmed-2009784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1978 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20097842009-09-10 Cell yield and cell survival following chemotherapy of the B16 melanoma. Stephens, T. C. Peacock, J. H. Br J Cancer Research Article We describe in this paper cell survival studies, using in vitro clonogenic assays, performed on the B16 melanoma treated in situ with various cytotoxic agents. In addition we have determined the effects of these agents on the yield of cells obtained by trypsinization. In untreated tumours the mean cell yield was approximately 10(8)/g, which is 20--30% of the cells actually present in the tissue. The plating efficiency was approximately 40%. Most agents rapidly affected both cell yield and cell survival. For example, within 20--30 h, gamma-radiation and several alkylating agents reduced cell yield by about 40%. The cell yield change was associated with an increase in mean cell size. Cell yield was reduced even more (approximately 70%) by Vinca alkaloids. This large reduction was associated with extensive cell lysis, observed as an increase in the necrotic fraction of tumours from approximately 35% to approximately 70%. Adriamycin, bleomycin and Ara-C also produced a moderate reduction in cell yield (approximately 40%), but actinomycin D did not reduce cell yield and FU increased it by about 30%. Only gamma-radiation, cyclophosphamide, CCNU, BCNU and melphalan produced more than a 90% reduction in cell survival, although there was a small but measurable reduction with all other agents except vinblastine, HN2 and actinomycin D. Nature Publishing Group 1978-11 /pmc/articles/PMC2009784/ /pubmed/728348 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Stephens, T. C. Peacock, J. H. Cell yield and cell survival following chemotherapy of the B16 melanoma. |
title | Cell yield and cell survival following chemotherapy of the B16 melanoma. |
title_full | Cell yield and cell survival following chemotherapy of the B16 melanoma. |
title_fullStr | Cell yield and cell survival following chemotherapy of the B16 melanoma. |
title_full_unstemmed | Cell yield and cell survival following chemotherapy of the B16 melanoma. |
title_short | Cell yield and cell survival following chemotherapy of the B16 melanoma. |
title_sort | cell yield and cell survival following chemotherapy of the b16 melanoma. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009784/ https://www.ncbi.nlm.nih.gov/pubmed/728348 |
work_keys_str_mv | AT stephenstc cellyieldandcellsurvivalfollowingchemotherapyoftheb16melanoma AT peacockjh cellyieldandcellsurvivalfollowingchemotherapyoftheb16melanoma |