Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.

Cells from the established cell line NHIK 3025 were synchronized by repeated mitotic selections. Survival of the synchronized cells after treatment with haematoporphyrin and near-UV light was measured by testing the capacity of the cells to form macroscopic colonies. The sensitivity to photodynamic...

Descripción completa

Detalles Bibliográficos
Autores principales: Christensen, T., Moan, J., Wibe, E., Oftebro, R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1979
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009795/
https://www.ncbi.nlm.nih.gov/pubmed/758929
_version_ 1782136187987689472
author Christensen, T.
Moan, J.
Wibe, E.
Oftebro, R.
author_facet Christensen, T.
Moan, J.
Wibe, E.
Oftebro, R.
author_sort Christensen, T.
collection PubMed
description Cells from the established cell line NHIK 3025 were synchronized by repeated mitotic selections. Survival of the synchronized cells after treatment with haematoporphyrin and near-UV light was measured by testing the capacity of the cells to form macroscopic colonies. The sensitivity to photodynamic inactivation was small in early G1, late S and G2. The sensitivity increased throughout late G1 and early S to a maximum in mid S. More than a 100-fold variation is found in the survival after 20 min irradiation in the presence of 4 X 10(-4)M haematoporphyrin.
format Text
id pubmed-2009795
institution National Center for Biotechnology Information
language English
publishDate 1979
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-20097952009-09-10 Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro. Christensen, T. Moan, J. Wibe, E. Oftebro, R. Br J Cancer Research Article Cells from the established cell line NHIK 3025 were synchronized by repeated mitotic selections. Survival of the synchronized cells after treatment with haematoporphyrin and near-UV light was measured by testing the capacity of the cells to form macroscopic colonies. The sensitivity to photodynamic inactivation was small in early G1, late S and G2. The sensitivity increased throughout late G1 and early S to a maximum in mid S. More than a 100-fold variation is found in the survival after 20 min irradiation in the presence of 4 X 10(-4)M haematoporphyrin. Nature Publishing Group 1979-01 /pmc/articles/PMC2009795/ /pubmed/758929 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Christensen, T.
Moan, J.
Wibe, E.
Oftebro, R.
Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.
title Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.
title_full Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.
title_fullStr Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.
title_full_unstemmed Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.
title_short Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.
title_sort photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line nhik 3025 cultivated in vitro.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009795/
https://www.ncbi.nlm.nih.gov/pubmed/758929
work_keys_str_mv AT christensent photodynamiceffectofhaematoporphyrinthroughoutthecellcycleofthehumancelllinenhik3025cultivatedinvitro
AT moanj photodynamiceffectofhaematoporphyrinthroughoutthecellcycleofthehumancelllinenhik3025cultivatedinvitro
AT wibee photodynamiceffectofhaematoporphyrinthroughoutthecellcycleofthehumancelllinenhik3025cultivatedinvitro
AT oftebror photodynamiceffectofhaematoporphyrinthroughoutthecellcycleofthehumancelllinenhik3025cultivatedinvitro

Ejemplares similares