ACTH-like activity in immune complexes of patients with oat-cell carcinoma of the lung.

Immune complexes could be isolated from sera of 7 patients with oat-cell carcinoma of the lung, but not from 5 normal controls, using zonal ultracentrifugation. After ultracentrifugation, fractions containing macromolecular IgG were absorbed on a protein A-sepharose column and the immune complexes w...

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Autores principales: Havemann, K., Gropp, C., Scheuer, A., Scherfe, T., Gramse, M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1979
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009814/
https://www.ncbi.nlm.nih.gov/pubmed/215184
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author Havemann, K.
Gropp, C.
Scheuer, A.
Scherfe, T.
Gramse, M.
author_facet Havemann, K.
Gropp, C.
Scheuer, A.
Scherfe, T.
Gramse, M.
author_sort Havemann, K.
collection PubMed
description Immune complexes could be isolated from sera of 7 patients with oat-cell carcinoma of the lung, but not from 5 normal controls, using zonal ultracentrifugation. After ultracentrifugation, fractions containing macromolecular IgG were absorbed on a protein A-sepharose column and the immune complexes were eluted and dissociated by glycin-HCl buffer at pH 3.5. The eluates were tested for the presence of tumour-associated proteins as carcinoembryonic antigen (CEA), non-specific crossreacting antigen (NCA), alpha2 pregnancy associated antigen (alpha2PAG) and isoferritin. Whereas none of these tumour-associated antigens could be demonstrated, an ACTH-like activity was detected in the immune-complex fractions of 4 patients with oat-cell carcinoma, by radioimmuno- and bioassay. Polyacrylamide electrophoresis of an immune-complex fraction from a patient with Cushing syndrome showed ACTH-like activities, with mol. wt of 110,000, 75,000, 30,000 and less than 20,000 (all glycoproteins) indicating the presence of different subfractions of big ACTH. IMAGES:
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spelling pubmed-20098142009-09-10 ACTH-like activity in immune complexes of patients with oat-cell carcinoma of the lung. Havemann, K. Gropp, C. Scheuer, A. Scherfe, T. Gramse, M. Br J Cancer Research Article Immune complexes could be isolated from sera of 7 patients with oat-cell carcinoma of the lung, but not from 5 normal controls, using zonal ultracentrifugation. After ultracentrifugation, fractions containing macromolecular IgG were absorbed on a protein A-sepharose column and the immune complexes were eluted and dissociated by glycin-HCl buffer at pH 3.5. The eluates were tested for the presence of tumour-associated proteins as carcinoembryonic antigen (CEA), non-specific crossreacting antigen (NCA), alpha2 pregnancy associated antigen (alpha2PAG) and isoferritin. Whereas none of these tumour-associated antigens could be demonstrated, an ACTH-like activity was detected in the immune-complex fractions of 4 patients with oat-cell carcinoma, by radioimmuno- and bioassay. Polyacrylamide electrophoresis of an immune-complex fraction from a patient with Cushing syndrome showed ACTH-like activities, with mol. wt of 110,000, 75,000, 30,000 and less than 20,000 (all glycoproteins) indicating the presence of different subfractions of big ACTH. IMAGES: Nature Publishing Group 1979-01 /pmc/articles/PMC2009814/ /pubmed/215184 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Havemann, K.
Gropp, C.
Scheuer, A.
Scherfe, T.
Gramse, M.
ACTH-like activity in immune complexes of patients with oat-cell carcinoma of the lung.
title ACTH-like activity in immune complexes of patients with oat-cell carcinoma of the lung.
title_full ACTH-like activity in immune complexes of patients with oat-cell carcinoma of the lung.
title_fullStr ACTH-like activity in immune complexes of patients with oat-cell carcinoma of the lung.
title_full_unstemmed ACTH-like activity in immune complexes of patients with oat-cell carcinoma of the lung.
title_short ACTH-like activity in immune complexes of patients with oat-cell carcinoma of the lung.
title_sort acth-like activity in immune complexes of patients with oat-cell carcinoma of the lung.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2009814/
https://www.ncbi.nlm.nih.gov/pubmed/215184
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